118 



MANUAL or DETERMINATIVE BACTERIOLOGY 



Kjusu Imp. Univ., 4, 1931, 545 has not 

 been seen. 



78. Pseudomonas marginata (McCul- 

 loch) Stapp. {Bacterium viarginatum 

 McCulloch, Science, 5If, 1921, 115; Jour. 

 Agr. Res., 29, 1924, 174; Phytomon'as 

 marginata Bergey et al.. Manual, 1st ed., 

 1923, 188; Stapp, in Sorauer, Handbuch 

 der Pflanzenkrankheiten, 2, 5 Auf., 1928, 

 56.) From L. marginatus, having a 

 border, probably refers to the definite 

 margin of the colony. 



Rods: 0.5 to 0.6 by 0.8 to 1.8 microns. 

 Motile with 1 to 4 bipolar flagella. Cap- 

 sules. Gram-negative. 



Green fluorescent pigment produced in 

 Uschinsky's and Fermi's solutions. 



Gelatin: Liquefied. 



Agar colonies : White, circular, smooth, 

 translucent, viscid, with definite margins 

 at first thin but later thick and con- 

 toured. Surface wrinkled. 



Milk: At first slightly acid, then alka- 

 line. Casein digested. 



Nitrites not produced from nitrates. 



Indole production slight. 



Hydrogen sulfide production slight. 



Lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but not gas from glucose, lactose, 

 sucrose and glycerol. 



Starch hydrolysis feeble. 



Growth in 3.5 per cent salt. No growth 

 in 4 per cent salt. pH range, 4.6 to 9.1. 



Optimum temperature 30° to 32°C. 

 Maximum 40°C. Minimum 8° to 9°C. 



Source : Repeatedly isolated from 

 diseased gladiolus. 



Habitat: Pathogenic on Gladiolus spp. 

 and Iris spp. 



79. Pseudomonas medicaginis Sackett. 

 (Sackett, Science, 31, 1910, 553; also 

 Colorado Agr. Exp. Sta., Bull. 158, 1910, 

 11 ; Bacillus medicaginis Holland, Jour. 

 Bact., 5, 1920, 219; Phytomonas medi- 

 caginis Bergey et al., Manual, 1st ed., 

 1923, 179; Bacterium medicaginis Elliott, 

 Bact. Plant Path., 1930, 162.) From L. 



medica, ancient Media; M.L. Medicago, a 

 generic name. 



Rods : 0.7 by 1 .2 microns. Motile with 

 1 to 4 flagella. Filaments present. 

 Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Not liquefied. 



Nutrient agar colonies: Growth in 24 

 hours whitish, glistening. 



Broth: Turbid in 24 hours. Pellicle 

 formed. Viscid sediment. 



Milk: Becomes alkaline. No change. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Starch not hydrolyzed. 



No gas from carbohydrates. Acid from 

 sucrose. 



Slight growth in broth plus 3.75 per cent 

 salt. 



Optimum temperature 28° to 30°. 

 Maximum 37.5°C. 



Aerobic. 



Source : Isolated from brown lesions on 

 leaves and stems of alfalfa. 



Habitat : Pathogenic on alfalfa, Medi- 

 cago sp. 



79a. Pseudomonas phaseolicola (Burk- 

 holder) Dowson. {Phytomonas medi- 

 caginis var. phaseolicola Burkholder, 

 Phytopath., 16, 1926, 915; Bacterium 

 medicaginis var. phaseolicola Link and 

 Hull, Bot. Gaz., 83, 1927, 413; Pseudo- 

 monas medicaginis var. phaseolicola 

 Stapp and Kotte, Nachrichtenb. f. d. 

 Deutschen Pflanzenschutzdienst, 9, 1929, 

 35; Dowson, Brit. Mycol. Soc. Trans., 

 26, 1943, 10.) From L. phaseolus, bean; 

 M.L. Phaseolus, a generic name;-coZa, 

 dweller. 



Synonym : Bacterium puerariae 

 Hedges. (Phytopath., 17, 1927, 48 and 

 20, 1930, 140; Phytomonas puerariae 

 Bergey et al.. Manual, 3rd ed., 1930, 267.) 



Description from Burkholder and 

 Zaleski (Phytopath., 22, 1932, 85). 



