FAMILY PSEUDOMONADACEAE 



119 



Rods : 1 by 2 microns, sometimes 

 slightly curved, filaments present. 

 Motile with polar flagellum. Gram- 

 negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin stab: Slow liquefaction. 



Beef extract agar: Whitish, circular 

 colonies, 2 mm. in diameter. Edges 

 entire. 



Broth: Turbid. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



Hydrogen sulfide not formed. 



Not lipolytic (Starr and Burkholder, 

 Photopath., 35, 1942, 601). 



Acid but no gas from glucose, fructose, 

 mannose, arabinose, xjdose, sucrose and 

 glycerol. No acid from rhamnose, lac- 

 tose, maltose, mannitol and salicin. 

 Alkali from salts of citric and malic acids, 

 but not from acetic, formic, lactic or 

 tartaric acids. Starch and cellulose not 

 hydrolyzed. 



Slight growth in broth plus 4 per cent 

 salt. 



Optimum temperature 20"" to 23°C. 

 Maximum 33°C. Minimum 2.5°C. 

 (Hedges, loc. cit.). 



Optimum pH 6.7 to 7.3. Maximum S.8 

 to 9.2. Minimum 5.0 to 5.3. (Kotte, 

 Phyt. Zeitsch., 2, 1930, 453.) 



Microaerophilic. 



Source : Isolated from leaves, pod and 

 stem of beans showing halo blight. 



Habitat: Pathogenic on beans (Phaseo- 

 lus vulgaris), the kudzu vine (Pueraria 

 hirsula) and related plants. 



80. Pseudomonas pisi Sackett. (Sack- 

 ett, Colorado Agr. E.xp. Sta., Bull. 218, 

 1916, 19; Bacterium -pisi Erw. Smith, 

 An Introduction to Bacterial Diseases of 

 Plants, 1920, 474; Phytomonas pisi Ber- 

 gey et al.. Manual, 1st ed., 1923, 181.) 

 From Gr. pisum, the pea; M.L. Pisum, 

 a generic name. 



Rods : 0.68 to 2.26 microns. Motile 

 with a polar flagellum. Gram -negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction. 



Agar slants : Moderate growth in 24 

 hours, filiform, glistening, grayish-white. 



Broth: Turbid with a scum in 5 days. 



Milk: Alkaline, soft curd, clears. 



Nitrites not produced from nitrates. 



Indole not produced! 



No H2S produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but notgas from glucose, galactose 

 and sucrose. 



Starch not hydrolyzed. 



Optimum temperature 27" to 28°C. 

 Maximum 37.5°C. Minimum 7°C. 



Aerobic. 



Source : Ten cultures isolated from 5 

 collections of diseased peas showing water 

 soaked lesions on stems and petioles. 



Habitat: Pathogenic on garden peas, 

 Pisum sativum and field peas, P. sativum 

 var. arvense. 



81. Pseudomonas syringae van Hall. 

 (Kennis der Bakter. Pfianzenziekte, 

 Inaug. Diss., Amsterdam, 1902, 191; 

 Bacterium syringae Erw. Smith, Bacteria 

 in Relation to Plant Diseases, 1, 1905, 68; 

 Phytomonas syringae Bergey et al., 

 Manual, 3rd ed., 1930, 257.) From 

 Latin, syringa, a nymph that was 

 changed into a reed; M.L. Syringa, a 

 generic name. 



Synonyms: Bryan (Jour. Agr. Res., 38, 

 1928, 225) lists Bacterium citriputeale C. 

 O. Smith, Phytopath., 3, 1913, 69, and 

 Bacterium citrarefaciens Lee, Jour. Agr. 

 Res., 9, 1917, 1 {Pseudomonas citrare- 

 faciens Stapp, in Sorauer, Handb. d. 

 Pflanzenkrankheiten, 2, 5 Aufl., 1928, 

 190). Clara (Cornell Agr. Exp. Sta. 

 Mem. 159, 1934, 29) lists Bacterium vignae 

 {Pseudomonas vignae) Gardner and Ken- 

 drick, Science, 57, 1923, 275 {Phytomonas 

 vignae Bergey et al., Manual, 1st ed., 

 1923, 188), Pseudomonas viridifaciens 

 Tisdale and Williamson, Jour. Agr. Res., 

 25, 1923, 141 .{Bacterium viridifaciens 

 Tisdale and Williams, ibid. ; Phytomonas 



