FAMILY PSEUDOMONADACEAE 



121 



McCulloch, Jour. Agr. Res., 18, 1920, 

 549 ; Phytomonas atrofaciens Bergey et 

 al., Manual, 1st ed., 1923, 185; Stevens, 

 Plant Disease Fungi, New York, 1925, 22.) 

 From Latin, ater, black; faciens, making, 

 referring to the color of the lesion on 

 wheat. 



Rods: 0.6 by 1 to 2.7 microns. Long 

 chains formed in culture. Capsules 

 present. Motile with 1 to 4 polar or bi- 

 polar flagella. Gram -negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: Liquefied. 



Beef-peptone-agar colonies: Circular, 

 shining, translucent, white. 



Broth: Growth never heavy, slight 

 rim, and a delicate pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole: Slight production. 



Hj^drogen sulfide: Slight production. 



Acid and no gas from glucose, galac- 

 tose and sucrose. 



Starch is slightly hytlrolyzed. 



Optimum temperature 25° to 28°C. 

 Maximum 36° to 37°C and minimum be- 

 low 2°C. 



Aerobic. 



Sources : Isolated from diseased wheat 

 grains collected throughout United States 

 and Canada. 



Habitat: Causes a basal glume-rot of 

 wheat. 



83. Pseudomonas cumini (Kovacev- 

 ski) Dowson. {Phytomonas cumini Kov- 

 acevski. Bull. Soc. Bot. Bulgarie, 7, 1936, 

 27; Dowson, Trans. Brit. Mycol. Soc, 

 26, 1943, 10.) From Greek cuminum, 

 cumin; M.L. Cuminum, a generic name. 



Rods : 0.5 to 0.7 by 1 to 3 microns, oc- 

 curring in chains and filaments. Motile 

 with 1 to 3 polar flagella. Gram-negative. 



Green fluorescent pigment formed in 

 culture. 



Gelatin: Rapidly liquefied. 



Potato agar colonies: Grayish-white, 

 circular, glistening, smooth, butyrous. 



Broth: Moderate turbidity. Pseudo- 

 zoogloea. 



Milk: Xot coagulated. Casein pep- 

 tonized. 



Nitrites not produced from nitrates. 



Indole not formed. 



No HaS formed. 



Acid but not gas from glucose and 

 sucrose. No acid from lactose or glyc- 

 erol. Starch not hydrolyzed. 

 ■' Temperature range 5°C to 31°C. 



Aerobic. 



Source : Isolated from blighted cumin 

 (Cuminum). 



Habitat : Pathogenic on cumin and dill. 



84. Pseudomonas desaiana (Burk- 

 holder) comb. nov. (B. pyocyancus sac- 

 charum Desai, Ind. Jour. Agr. Sci., 5, 

 1935, 391 ; Phytomomas desaiana Burk- 

 holder, in Bergey et al., Manual, 5th ed., 

 1939, 174.) Named for Desai who first 

 isolated the species. 



Rods: 0.6 to 1.2 by 1.2 to 2.2 microns. 

 Motile with a polar flagellum. Gram- 

 negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: Liquefaction. 



Agar colonies: Grayish-blue. Raised. 



Broth: Light clouding. Pellicle. 



Milk: Peptonized without coagulation. 



Nitrites not produced from nitrates. 



Indole not formed. 



Glucose, sucrose, lactose and glycerol 

 fermented without gas. 



Starch: Hydrolysis present. 



Optimum temperature 30°C. 



Aerobic. 



Source : Isolated from stinking rot of 

 sugar cane in India and associated with a 

 white non-pathogenic bacterium. 



Habitat : Pathogenic on sugar cane, 

 Saccharu tn officinar urn . 



85. Pseudomonas erodii Lewis. (Phy- 

 topath., 4, 1914, 231; Bacterium erodii 

 Lewis, ibid.; Phytomonas erodii Bergey 

 et al., ^'lanual, 3rd ed., 1930, 256.) From 

 Greek, erodius, heron; ]\I.L. Erodium, 

 a generic name. 



Rods: 0.6 to 0.8 by 1.2 to 1.8 microns. 



