122 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Motile with 1 to 3 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: Liquefaction. 



Agar stroke : Heavy, smooth, cream - 

 colored growth in 24 hours. 



Broth: Dense clouding in 24 hours. 



Milk : Turns alkaline and clears, litmus 

 reduced. 



Nitrites not produced from nitrates. 



Indole produced in 14 days. 



No H2S produced. 



Acid but not gas from glucose, sucrose, 

 lactose and glycerol. 



Temperature: No growth at 35°C. 



Aerobic, obligate. 



Source: Isolations from Erodium tex- 

 anum and 4 varieties of Pelargonium. 



Habitat : Causes a leaf spot of Erodium 

 iexanum and Pelargonium spp. 



86. Pseudomonas apii Jagger. (Jagger, 

 Jour. Agr. Res., £1, 1921, 186; Phyto- 

 monas apii Bergey et al.. Manual, 1st ed., 

 1923, 184; Pseudomonas jaggeri Stapp, in 

 Sorauer, Handbuch der Pflanzenkrank- 

 heiten, 2, 5 Aufl., 1928, 210; Bacterium 

 jaggeri Elliott, Bacterial Plant Patho- 

 gens, 1930, 142; Phytomonas jaggeri 

 Magrou, in Handuroy et al., Diet. d. 

 Bact. Path., Paris, 1937, 371.) From 

 Latin, apium, parsley, M.L. Apium, a 

 generic name. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 159, 1934, 24). 



Rods: 0.75 to 1.5 by 1.5 to 3.0 microns. 

 Motile with a polar flagellum. Gram- 

 negative. 



Green fluorescent pigment produced 

 in various media. 



Gelatin: Liquefaction. 



Beef -extract agar colonies: Circular, 

 glistening, smooth, edges entire. Gray- 

 ish-white with bluish tinge. 



Broth: Turbid in 36 hours. PeUicle 

 formed. 



Milk: Becomes alkaline. No curd. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S formed. 



Acid but not gas from glucose, galac- 

 tose, fructose, mannose, arabinose, 

 xylose, sucrose, mannitol and glycerol. 

 Alkaline reaction from salts of acetic, 

 citric, malic and succinic acids. Rham- 

 nose, maltose, lactose, raffinose, salicin, 

 and formic, lactic and tartaric acid are 

 not utilized. 



Starch not hydrolyzed. 



Facultative anaerobe. 



Distinctive characters : Pathogenicity 

 appears limited to celery. 



Source : Jagger isolated this repeatedly 

 from diseased celery leaves. 



Habitat: Pathogenic on celery, Apium 

 graveolens. 



87. Pseudomonas matthiolae (Briosi 

 and Pavarino) Dowson. {Bacterium mat- 

 thiolae Briosi and Pavarino, Atti della 

 Reale Accad. dei Lincei Rend., 21 , 1912, 

 216; Phytomonas matthiolae, Bergey et 

 al.. Manual, 3rd ed., 1930, 266; Mushin, 

 Proc. Roy. Soc. Victoria, 53, 1941, 201; 

 Dowson, Trans. Brit. Mycol. Soc, 26, 

 1943, 10.) From M.L. Matthiola, a 

 generic name. 



Rods: 0.4 to 0.6 by 2 to 4 microns. 

 Gram-positive. Gram-negative (Mush- 

 in, loc. cit.). 



Green fluorescent pigment produced 

 in culture. 



Gelatin : Liquefied. 



Beef agar colonies : White, circular 

 colonies, slightly elevated, margins 

 smooth. 



Broth: Slightly turbid. Becomes pale 

 green. 



Milk: Coagulation with acid reacfon. 



Nitrites produced from nitrates 

 (Mushin). 



Hydrogen sulfide not formed. 



Acid from glucose, galactose, fructose, 

 mannose, rhamnose, glycerol, mannitol, 

 acetic acid, citric acid, formic acid, lactic 

 acid, malic acid, and succinic acid. 

 Feeble acid in maltose. No acid, no gas 

 in lactose, sucrose, raffinose, starch, 

 salicin, and tartaric acid (Mushin). 



Optimimi temperature 20 to 24°C. 



