FAMILY PSEUDOMONADACEAE 



123 



Maximum temperature 38.5 C. Mini- 

 mum below 0°C. (Mushin). 



Limits of growth in broth are pH 4.4 

 to pH 9.5 (Mushin). 



Aerobic. 



Source: Isolated from vascular and 

 parenchymatic disease of stocks, Mat- 

 thiola incana var. annua. 



Habitat: Pathogenic on stocks. 



Note: Burkholder (Phytopath., 28, 

 1938, 936) and Santarelli (Rev. di Pat. 

 Veg., 29, 1939, 364) consider this species 

 a synonym of Pseudomonas syringae. 

 Adam and Pugsley (Jour. Dept. Agric. 

 Victoria, 32, 1934, 306) give a descrip- 

 tion of a green fluorescent pathogen on 

 stocks which is similar to Pseudomonas 

 syringae. Mushin {loc. cit.) considers 

 Pseudomonas matthiolae to be a distinct 

 species. 



88. Pseudomonas mors-prunorum 

 Wormald. (Jour. Pom. and Hort. Sci., 

 9, 1931,251; Phytomonas mors-prunorum 

 Wormald, Trans. Brit. Mycol. Soc, 17, 

 1932, 169; Bacterium mors-prunorum, 

 ibid.) From L. mors, death; prunus, 

 plums. 



Rods: Motile with a polar flagellum. 

 Gram-positive (1931). Gram-negative 

 (1932). 



Note : Possibly a green fluorescent or- 

 ganism since it produces a faint yellow 

 color in Uschinsky's solution. 



Gelatin: Liquefaction. 



Agar colonies : White. 



Broth plus 5 per cent sucrose: White 

 and cloudy. 



Nitrites not produced from nitrates. 



Acid but not gas from glucose, lactose, 

 sucrose and glycerol. 



Starch not hydrolyzed. 



Strict aerobe. 



Distinctive characters : Differs from 

 Pseudomonas prunicola {Pseudomonas 

 syringae) in that it produces a white 

 cloudy growth in broth plus 5 per cent 

 sucrose; a rapid acid production in nu- 

 trient agar plus 5 per cent sucrose, and a 

 faint yellow or no color in Uschinsky's 

 solution. 



Source : Isolated from cankers on plum 

 trees in England. 

 Habitat : Pathogenic on Prunus spp. 



89. Pseudomonas rimaefaciens Koning. 



(Chron. Bot., 4, 1938, 11 ; Meded. Phytop. 

 Labor, Willie Comm. Scholt., 14, 1938, 

 24.) From L. rima, fissure; Jaciens, pro- 

 ducing. 



Rods: 0.6 to 2.4 microns in length. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative. 



Yellow -green fluorescent water-soluble 

 pigment produced in culture. 



Gelatin : Liquified. 



Agar colonies: Round, convex, smooth, 

 somewhat granular with hyaline edge. 



Broth : Turbid. Surface growth with 

 a sediment in a few days. 



Milk: Alkaline and clears. 



Nitrites not produced from nitrates. 

 Peptone, asparagin, urea, gelatin, ni- 

 trates and ammonia salts are sources of 

 nitrogen. 



Hydrogen sulfide not produced. 



Indole production slight. 



Growth with the following carbon 

 sources plus NO3, glucose, sucrose, 

 glycerol, succinates, malates, citrates 

 and oxalates. Less growth with manni- 

 tol, fructose, galactose, lactose, salicy- 

 late. Acid is produced from the sugars. 

 No growth with dextrin, inulin, maltose, 

 lactose, rhamnose, salicin, tartrates, 

 acetates, formates. 



Starch not hj^drolyzed. 



Aerobic. 



Optimum temperature 25°C. Maxi- 

 mum about 37°C. Very slow growth at 

 14°C. Thermal death point 42° to 48°C. 



Source: Strains of the pathogen iso- 

 ated from poplar cankers in France and 

 in the Netherlands. 



Habitat : Pathogenic on Populus bra- 

 bantica, P. trichocarpa and P. candicans. 



This may be Pseudomonas syringae 

 since the characters are the same and 

 both organisms can infect Impatiens sp. 

 Pseudomonas syringae infects poplars 

 (Elliott, Bacterial Plant Pathogens, 

 1930, 218). 



