FAMILY PSEUDOMONADACEAE 



125 



Gelatin: Liquefied (Burkholder). 



Acid but no gas is produced from 

 glucose, sucrose, maltose, lactose, glyc- 

 erine, arabinose, xylose, galactose, raf- 

 finose and mannitol. 



Slight growth in broth plus 5 per cent 

 salt (Burkholder). 



Source : Isolated from stalk rot of field 

 corn in California; also from Diabrotica 

 beetles. 



Habitat : Pathogenic on corn and sugar 

 cane. 



Note : Like Pseudomonas desiana. 



94. Pseudomonas bowlesiae (Lewis 

 and \Yatson) Dowson. (Phytomonas 

 boidesii Lewis and Watson, Phytopath.. 

 17, 1927, 511; Bacterimn bowlesii Elliott, 

 Bacterial Plant Pathogens, 1930, 9G; 

 Dowson, Trans. Brit. IMycol. Soc, 26, 

 1943, 9.) From 'SI. L. Boidcsia. a generic 

 name. 



Rods: 0.5 to 0.7 by 1.2 to 1.6 microns, 

 occurring singl}^ in pairs or in short 

 chains. Motile with bipolar flagella. 

 Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin : Liquefied. 



Agar slants: Yellowish, moist, glisten- 

 ing and viscid. 



Broth: Uniform turbidity- throughout. 

 Heavy viscous sediment in old cultures. 



Milk: Alkaline; coagulation, with a 

 slow peptonization. 



Nitrites are produced from nitrates. 



Indole is produced. 



Hydrogen sulfide is produced. 



Acid from glucose, maltose and xylose. 

 No acid from sucrose. 



Optimum temperature 27"C. Maxi- 

 mum 37°C. Minimum — l^C. 



Optimum pH 7.2. pH range 4.5 to 8.6. 



Aerobic. 



Source: Isolated from diseased, water 

 soaked spots of bowlesia. 



Habitat : Pathogenic on Bowlesia sep- 

 tentrionalis. 



95. Pseudomonas intybi (Swingle) 

 Stapp. {Phytomonas intybi Swingle, 



Phytopath., 15, 1925, 730; Stapp, in Sor- 

 aurer, Handbuch der Pflanzenkrank- 

 heiten, 2, 5 Auf., 1928, 291; Bacterium 

 intybi Elliott, Bacterial Plant Pathogens, 

 1930, 142.) From Latin, intibus, endive. 



Description from Stapp, Cent. f. Bakt., 

 II Abt., 9/, 1935, 232. 



Rods: 0.4 to 0.5 by 1.4 to 2.8 microns. 

 Motile with one to several polar flagella. 

 Gram-negative. 



Green fluorescent pigment formed in 

 culture. 



Gelatin: Liquefaction. 



Agar colonies : White, glistening, trans- 

 parent. 



Broth: Turbid with fragile pellicle, 

 and good sediment. 



Milk: Coagulated. Casein not pep- 

 tonized. 



Nitrites are produced from nitrates 

 with the formation of gas. 



Indole not formed. 



Acid but not gas from arabinose, xylose 

 and glucose. No acid from sucrose. 



Optimum temperature 23° to 28°C. 

 Maximum 40° to 42°C. Minimum 0°C. 



Distinctive character: Differs from 

 Psexidomonas cichorii in that it liquefies 

 gelatin and produces nitrites from ni- 

 trates. 



Source : Isolated from French endive, 

 Cichorium inlybus by Swingle, from C. 

 endiva and lettuce, Lactuca saliva by 

 Stapp. 



Habitat : Pathogenic on endive and let- 

 tuce, causing a rot. 



96. Pseudomonas marginalis (Brown) 

 Stevens. (Bacterium marginale Brown, 

 Jour. Agr. Res., 13, 1918, SSQ;Phylo}nonas 

 marginalis Bergey et al.. Manual, 1st ed., 

 1923, 182; Stevens, Plant Disease Fungi, 

 New York, 1925, 30.) From Latin, margo 

 {marginis), edge, margin; M.L. viar- 

 ginalis, on the margin, a character of the 

 disease. 



Description from Brown (loc. cit.) and 

 Clara (Cornell Agr. Exp. Sta. Mem. 159, 

 1934, 27). 



Rods : Motile with 1 to 3 polar flagella. 

 Gram-negative. 



