126 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction. 



Agar colonies: Cream-colored to yel- 

 lowish. 



Brotla: Turbid, with pellicle. 



Milk: Alkaline. Soft curd at times. 



Nitrites are produced from nitrates. 

 Not produced (Clara, loc. cit.). 



Indole not produced. 



No H2S produced. 



Acid but not gas from glucose, galac- 

 tose, fructose, mannose, arabinose, xylose, 

 rhamnose, mannitol and glycerol. Al- 

 kali from salts of acetic, citric, malic, 

 formic, lactic, succinic and tartaric acid. 

 Sucrose, maltose, lactose, raffinose and 

 salicin not fermented (Clara, loc. cit.). 



Starch hydrolysis feeble. None (Clara, 

 loc. cit.). 



Optimum temperature 25° to 26°C. 

 Maximum 38°C. Minimum 0°C. 



Aerobic. 



Source : Isolated from marginal lesion 

 on lettuce from Kansas. 



Habitat : Pathogenic on lettuce and 

 related plants. 



97. Pseudomonas setariae (Okabe) 

 comb. nov. (Bacterium setariae Okabe, 

 Jour. Soc. Trop. Agr. Formosa, 6, 1934, 

 63; Phytomonas setariae Burkholder, in 

 Bergey, Manual, 5th ed., 1939, -183.) 

 From L. seia, bristle; -arius, like; M. L. 

 Setaria, a generic name. 



Rods: 0.4 to 0.8 by 1.8 to 4.4 microns. 

 Motile with a polar, seldom bipolar, flagel- 

 lum. Gram-negative. 



Yellowish water-soluble pigment pro- 

 duced in culture. 



Gelatin: Slow liquefaction. 



Beef -extract agar colonies : Circular, 

 white, opalescent, smooth, glistening. 



Broth: Turbid after 18 hours. Pel- 

 licle. 



Milk: Alkaline; clears. 



Nitrites are produced from nitrates. 



Indole is produced. 



No H2S produced. 



Acid but not gas from glucose, galac- 



tose and glycerol. No acid from lactose, 

 maltose or sucrose. 



Starch: Feeble hydrolysis. 



Grows in 3 per cent salt. 



Optimum temperature 31° to 34°C. 

 Maximum 42°C. 



Aerobic. 



Source : Isolated from brown stripe of 

 Italian millet. 



Habitat: Pathogenic on Italian millet, 

 Setaria italica. 



98. Pseudomonas polycolor Clara. 

 (Clara, Phytopath., 20, 1930, 704; Phyto- 

 monas polycolor Clara, ibid., Bacterium 

 polycolor Burgwitz, Phytopathogenic 

 Bacteria, Leningrad, 1935, 148.) From 

 Gr. poly, many; L. color, color. 



Note: Delacroix (Comp. rend. Acad. 

 Sci., Paris, 137, 1903, 454) describes 

 Bacilhis aerogenosus as being a tobacco 

 pathogen. The organism described by 

 Delacroix might be the same as Pseudo- 

 monas polycolor. Braun and Elrod 

 (Jour. Bact., 4S, 1942, 40) are of the 

 opinion that Clara's pathogen is Pseudo- 

 monas aeruginosa. 



Description taken from Clara (Cornell 

 Agr. Exp. Sta. Mem. 159, 1934, 28). 



Rods : 0.75 to 1 .2 by 1.05 to 3.0 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction. 



Beef-extract agar colonies: Gra3ash- 

 white, circular, raised, thin transparent 

 margins. 



Broth: Turbid in 36 hours with thin 

 pellicle. 



Milk: Alkaline; no curd. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Lipolytic (Starr and Burkholder, Phy- 

 topath., 5^, 1942,601). 



Acid but not gas from glucose, galac- 

 tose, fructose, mannose, arabinose, xy- 

 lose, mannitol and glycerol. Alkaline 

 reaction from salts of acetic, citric, malic, 



