FAMILY PSEUDOMONADACEAE 



127 



lactic and formic acid. Rhamnose, su- 

 crose, maltose, lactose, raffinose and sali- 

 cin not fermented . 



Starch not hydrolyzed. 



Facultative anaerobe. 



Good growth in broth plus 7 per cent 

 salt. 



Optimum temperature 25" to 30°C. 

 Maximum 37° to 39°C. 



Distinctive character: Differs from 

 Pseudomonas mellea in tj'pe of lesion pro- 

 duced, does not digest starch, nor reduce 

 nitrates and does not form acid from lac- 

 tose nor sucrose. Pathogenic for labora- 

 tory animals (Elrod and Braun, Sci. 94, 

 1941, 520). 



Source : Repeatedly isolated from leaf 

 spot of tobacco in the Philippines. 



Habitat: Pathogenic on tobacco. 



99. Pseudomonas viridiflava (Burk- 

 holder) Clara. {Phytomonas viridiflava 

 Burkh., Cornell Agr. Exp. Sta. Mem. 127, 

 1930, 63; Clara, Science, 75, 1934, 111; 

 Bacterium viridiflavum Burgwitz, Phyto- 

 pathogenic Bacteria, Leningrad, 1935, 

 127.) From Latin viridis, green; flaviis, 

 yellow. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 139, 1934, 30). 



Rods: 0.75 to 1.5 by 1.5 to 3.15 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction. 



Beef -extract agar colonies : Grayish- 

 white, margins corrugated, edges irregu- 

 lar. 



Broth: Turbid in 36 hours. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 82, 1942, 601). 



Acid but not gas from glucose, fruc- 

 tose, mannose, arabinose, xylose, manni- 

 tol and glycerol. Alkaline reaction from 

 salts of acetic, citric, malic, lactic and 



succinic acids. Sucrose, lactose, mal- 

 tose, raffinose, salicin, and salts of formic 

 and tartaric acids not fermented. 



Starch: No hydrolysis. 



Growth in broth plus 5 per cent NaCl. 



Facultative anaerobe. 



Source: Two cultures isolated from 

 spotted beans, one from England and 

 one from Switzerland. 



Habitat : Pathogenic on bean, Phaseo- 



lus vidgaris. 



99a. Pseudomonas viridiflava var. con- 

 centrica (Petersen) corrib. nov. {Phyto- 

 monas viridiflava var. concentrica Peter- 

 sen, Tridsskr. f. Planteavl., 88, 1932, 851; 

 Bacterium viridiflavum var. conceniricum 

 Burgwitz, Phytopathogenic Bacteria, 

 Leningrad, 1935, 127.) From M. L. 

 concentricus, concentric, referring to 

 the rings on the colonies. 



Distinctive characters : Differs from 

 Pseudomonas viridiflava in that it does 

 not grow in Uschinsky's solution, and also 

 in the shape of the colonies. 



Source : Isolated from the stems and 

 leaves of blighted beans in Denmark. 



Habitat : Pathogenic on the bean, 

 Phaseolus vidgaris. 



100. Pseudomonas ananas Serrano. 

 (Serrano, Philipp. Jour. Sci., 55, 1934, 

 355; Phytomonas ananas and Bacterium 

 ananas Serrano, ibid, (not to be con- 

 fused with Erwinia ananas Serrano, 

 ibid., 86, 1928, 271); Bacterium serranoi 

 Burgwitz, Bact. Dis. of Plants, Lenin- 

 grad, 1936.) From Brazilian Indian, 

 ananas, pineapple; M.L. Ananas, generic 

 name . 



Rods : 0.6 by 1.8 microns. Motile with 

 1 to 4 polar flagella. Gram -negative. 



Green fluorescent pigment produced 

 in certain media. 



Gelatin: Liquefied. 



Beef-extract glucose agar colonies : 

 White, v^ith undulating edges, smooth to 

 rugose, glistening to dull. 



Beef -extract agar: Growth scant. 



Broth: Feeble growth. 



