128 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Milk: Becomes alkaline with curd. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S formed. 



Acid but not gas from glucose, xylose 

 and mannitol. Feeble with lactose. No 

 acid with sucrose. 



Starch not hydrolyzed. 



Optimum temperature 30° to 31°C. 

 Maximum 45°C. Minimum 7° to 10°C. 



Aerobic. 



Habitat : Causes a rot of pineapples, 

 Ananas comosus. 



101. Pseudomonas ligustri (d'Oliveira) 

 comb. nov. {Bacterium ligustri d'Oli- 

 veira, Revista Agron., 24, 1936, 434.) 

 From L. ligustrum, privet; M. L. Ligus- 

 trum, a generic name. 



Rods: 0.5 to 0.7 by 1.3 to 3 microns. 

 No chains. No capsules. Motile with 

 2 to 5 polar flagella. Gram-negative. 



Green pigment produced on Dox agar, 

 and in broth. 



Gelatin: Liquefied. 



Beef-extract agar colonies : Growth 

 moderate. Milky white, circular, convex. 



Broth: Turbid ift 24 hours. No pel- 

 licle. 



Milk : Coagulated in 6 days, and later 

 digested. Litmus slightly acid. 



Nitrites not produced from nitrates. 



Indole not produced. 



Ammonia not produced. 



No gas from carbohydrates. Acid from 

 glucose, galactose, arabinose and man- 

 nose. No acid from sucrose, maltose, 

 lactose, raffinose, mannitol and salicin. 



Source : From diseased Japanese privet 

 in Lisbon, Portugal. 



Habitat: Pathogenic on privet, Ligtis- 

 trum japonicum. 



102. Pseudomonas sesami Malkoff. 

 (Malkoff, Cent. f. Bakt., II Abt., 16, 

 1906, 665; Bacterium sesami Nakata, 

 Ann. Phyt. Soc. Japan, 2, 1930, 242; 

 Phytomonas sesami Kovachersky, Ann. 

 Univ. de Sofia, Fac. Agron., 8, 1930, 464.) 

 From Gr. sesamum, sesame; M. L. Sesa- 

 mum, a generic name. 



Synonym: Nakata (loc. cit.) lists Bac- 

 ierimn sesamicola Takimoto, Jour. Plant 

 Protect. Tokyo, 8, 1927, 433 {Phyto- 

 monas sesamicola Magrou, in Hauduroy 

 et al.. Diet. d. Bact. Path., 1937, 412). 



Description from Nakata {loc. cit.). 



Rods: 0.6 to 0.8 by 1.2 to 3.8 microns. 

 Motile with 2 to 5 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction rapid. 



Beef -agar colonies : Circular, flat, stri- 

 ate, smooth, entire margins, white. 



Broth: Growth rapid. No pellicle. 



Milk: Alkaline. No coagulation. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Acid but not gas from glucose. No 

 acid from lactose, sucrose or glycerol. 



Starch not hydrolyzed. 



Optimum temperature 30°C. Maxi- 

 mum 35°C. Minimum 0°C. 



Facultative anaerobe. 



Source : Isolated from brown spots on 

 leaves and stems of sesame. 



Habitat: Pathogenic on sesame. 



103. Pseudomonas tolaasii Paine. 

 (Paine, Ann. Appl. Biol., 6, 1919, 210; 

 Phytomonas tolaasi Bergey et al., Man 

 ual, 3rd ed., 1930, 259; Bacterium tolaasi 

 Elliott, Bacterial Plant Pathogens, 1930, 

 226.) Named for A. G. Tolaas who first 

 reported the species. 



Rods: 0.4 to 0.5 by 0.9 to 1.7 microns. 

 Motile with 1 to 5 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. , 



Gelatin: Liquefaction. 



Bouillon agar: Streak develops in 24 

 hours, dirty bluish-white, wet-shining 

 and slightly raised. 



Broth: Turbid in 24 hours. Pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole production slight. 



Acid but not gas from glucose. No 

 acid from lactose or sucrose. 



