130 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



from blighted pear blossoms. Doidge 

 received a culture from Barker. 



Habitat: Causes a blossom blight of 

 pear. 



107. Pseudomonas gladioli Severini. 

 (Severini, Annali d. Bot., Rome, 11, 

 1913, 420; Bacterium gladioli Elliott, 

 Bact. Plant Pathogens, 1930, 132; Phyto- 

 monas gladioli Magrou, in Hauduroy et 

 al., Diet. d. Bact. Path., Paris, 1937, 

 356.) From L. gladiolus, a little sword; 

 M. L. Gladiolus, a generic name. 



Rods: 0.6 by 2.3 to 2.8 microns. Mo- 

 tile with one or more polar flagella. 

 Gram-negative. 



A pale yellow water-soluble pigment 

 found, later orange. 



Gelatin colonies: Cream -colored, wart- 

 like. Rapid liquefaction. 



Milk: Coagulated and slowly pep- 

 tonized. 



Nitrites not produced from nitrates. 



Indole not formed. 



No gas. 



Aerobic. 



Optimum temperature 28° to 30°C. 



Habitat : Causes a corm rot of gladiolus 

 and other tubers. 



108. Pseudomonas mellea Johnson. 

 {Bacterium melleum Johnson, Jour. Agr. 

 Res., ^S, 1923,489; Johnson, loc. cit., 489; 

 Phytomonas mellea Bergey et al., Manual, 

 3rd ed., 1930, 254.) From L. melleus, of 

 or belonging to honey, the color of the 

 colonies. 



Rods: 0.6 by 1.8 microns. Capsules. 

 Motile with 1 to 7 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: Liquefied. 



Potato - glucose agar: Abundant 

 growth, smooth, glistening, viscid, honey- 

 colored. 



Broth: Turbid in 24 hours. Pellicle. 



Milk: Alkaline; clears. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S formed. 



Starch hydrolysis feeble. 



Growth inhibited by 4 per cent salt. 



Optimum temperature, 26° to 28°C. 

 Maximum 36°C. 



Facultative anaerobe. 



Distinctive character: Differs from 

 Pseudomonas pseudozoogloeae in that it 

 produces on tobacco a brown instead of a 

 black spot with a halo, is orange-yellow 

 in culture, and turns milk alkaline. 



Source: Isolated from brown rusty 

 spots on tobacco in Wisconsin. 



Habitat : Pathogenic on leaves of to- 

 bacco, Nicotiana tahacum. 



109. Pseudomonas betlis (Raguna- 

 than) comb. nov. {Bacterium betle 

 Ragunathan, Ann. Roy. Card., Pera- 

 deniya, Ceylon, 11, 1928, 51; Aplanobac- 

 ter betle Elliott, Bact. Plant Pathogens, 

 1930, 4; Phytomonas betlis Magrou, in 

 Hauduroy et al., Diet. d. Bact. Path., 

 Paris, 1937, 337.) From Malayan, belle, 

 betel, a kind of pepper, Piper betle. 



Rods: 0.5 by 1.5 to 2.5 microns, occur- 

 ring singly or in short chains. Non- 

 motile. Gram-negative. 



Green pigment formed in nutrient gela- 

 tin and in broth. 



Gelatin: Liquefaction. 



Bovril agar colonies: Honey -yellow, 

 circular at first, later echinulate. Raised, 

 smooth and shiny. 



Broth: Surface becomes cloudy in 2 

 days. Pellicle. 



No gas from lactose, maltose or sucrose. 



Starch is reduced. 



Aerobic. 



Source : Five cultures isolated from 

 leaf spots on the betel vine. 



Habitat : Pathogenic on the betel vine, 

 Piper betle. 



110. Pseudomonas panacis (Takimoto) 

 Dowson. {Bacterium panaxi Nakata 

 and Takimoto, Bui. Agr. Sta. Chosen, 5, 

 1922, 1 ; Phytomonas panaxi Magrou, in 

 Hauduroy et al.. Diet. d. Bact. Path., 

 Paris, 1937, 389; Dowson, Trans. Brit. 



