132 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



terium soyae var. japonicum Takimoto, 

 Jour. Plant Protect. Tokyo, U, 1927, 

 556; Bacterium glycineum var. japonicum 

 Elliott, Bact. Plant Pathogens, 1930, 136; 

 Phytomonas glycinea var. japonica Ma- 

 grou, in Hauduroy et al.. Diet. d. Bact. 

 Path., Paris, 1937, 358.) From M. L. 

 Japonicus, Japanese. 



Distinctive characters : Differs slightly 

 from Pseudomonas glycinea in size of cell, 

 length of chains, action in milk, and color 

 in media. Okabe (Jour. Soc. Trop. 

 Agr., Formosa, 5, 1933, 162) gives a de- 

 scription of the organism which leads one 

 to believe the differences are not great 

 enough to be varietal. 



Source : Isolated from leaf spots on soy 

 bean in Formosa. 



Habitat : Pathogenic on soy bean, Gly- 

 cine max. 



113. Pseudomonas savastanoi (Erw 

 Smith) Stevens. (Bacterium savastano 

 Erw. Smith, U. S. Dept. Agr. Plant Ind 

 Bull. 131, 1908, 31; Stevens, The Fungi 

 which Cause Plant Diseases, 1913, 33 

 Phytomonas savastanoi Bergey et al.. 

 Manual, 1st ed., 1923, 190.) Named for 

 F. Savastano, the Italian plant path- 

 ologist. 



Note: Smith (loc. cit.) lists and dis- 

 cards the following species since they were 

 either mixed cultures or names with no 

 descriptions : Bacterium oleae Arcangeli , 

 Istit. Bot. delle R. Univ. di Pisa, Ri- 

 cerche e Lavori, fasc. 1, 1886, 109; Bacil- 

 lus oleae tuberculosis Savastano, Atti. 

 R. Accad. Naz. Lincei Rend. CI. Sci. Fis., 

 Mat. e Nat., 5, 1889, 92; Bacillus pril- 

 lieuxianus Trevisan, I generi e le specie 

 delle Batteriacee, Milano, 1889, 19; 

 Bacillus oleae De Toni and Trevisan, in 

 Saccardo, Sylloge Fungorum, 8, 1889, 

 982. 



Description from Brown, Jour. Agr. 

 Res., 44, 1932, 711. 



Rods: 0.4 to 0.8 by 1.2 to 3.3 microns. 

 Motile with 1 to 4 polar flagella. Gram- 

 negative. 



Green fluorescent pigment found in 

 culture. 



Gelatin: No liquefaction. 



Beef agar colonies: White, smooth, 

 flat, glistening, margins erose or entire. 



Broth : Turbid on the second day. No 

 pellicle or ring. 



Milk: Becomes alkaline. 



Nitrites not produced from nitrates. 



No H2S produced. 



Acid but not gas from glucose, galac- 

 tose and sucrose. 



Starch is hydrolyzed. 



Optimum temperature 23° to 24°C. 

 Maximum 32°C. Minimum TC. 



Optimum pH 6.8 to 7.0. Maximum 8.5. 

 Minimum 5.6. 



Aerobic. 



Source : Smith isolated his cultures 

 from olive galls collected in California. 



Habitat: Pathogenic on olive. 



113a. Pseudomonas savastanoi var. 

 fraxini (Brown) Dowson. (Bacterium 

 savastanoi var. fraxini Brown, Jour. 

 Agr. Res., 44, 1932, 721; Phytomonas 

 savastanoi var. fraxini Magrou, in Hau- 

 duroy et al., Diet. d. Bact. Path., Paris, 

 1937, 410; Pseudomonas fraxini Skoric, 

 Ann. Exp. For. Zagreb, 6, 1938, 66; Dow- 

 son, Trans. Brit. Mycol. Soc, 26, 1943, 

 11.) From M. L. Fraxinus, a generic 

 name. 



Distinctive characters: Differs but 

 slightly from Pseudomonas savastanoi, 

 but is pathogenic on ash and not on olive. 



Source : Three cultures isolated from 

 cankers on ash. 



Habitat: Pathogenic on ash, Fraxinus 

 excelsior and F. americana. 



114. Pseudomonas tonelliana (Fer- 

 raris) comb. nov. (Bacterium toncllianum 

 Ferraris, Trattato di Patologia e Terapia 

 Vegetale, 3rd ed., 1, 1926, 104; Phytomo- 

 nas tonelliana Adams and Pugsley, Jour. 

 Dept. Agr. Victoria, 32, 1934, 304.) 

 Named for Tonelli, the Italian botanist. 



Synonym : Pseudomonas savastanoi 

 var. ncrii C. O. Smith, Phytopath., 18, 

 1928, 503. 



Description from Smith (loc. cit.) un- 

 less otherwise noted 



