FAMILY PSEUDOMOXADACEAE 



133 



Rods: 0.5 to 0.6 by 1.5 to 2.5 microns. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative (Adams and Pugsley, loc. cil.). 



Gelatin: No liquefaction. 



Potato glucose agar colonies: Flat, 

 circular, shining, margins somewhat un- 

 dulated. 



Broth: Dense clouding with partial 

 pellicle. 



Milk: Alkaline. No separation. 



Nitrites not produced from nitrates 

 (Adams and Pugsley). 



Indole produced. Not produced (Adams 

 and Pugsley). 



Acid but not gas from glucose and 

 sucrose. No acid from lactose (Adams 

 and Pugsley). 



Starch not hydrolyzed (Adams and 

 Pugsley). 



Distinctive character : Pseudomonas 

 savastanoi is similar in culture but is not 

 pathogenic on oleanders. 



Source: Both Ferraris and C. O. Smith 

 isolated the pathogen from galls on olean- 

 der. 



Habitat: Pathogenic on oleander, 

 Nerium oleander. 



115. Pseudomonas calendulae (Taki- 

 moto) Dowson. {Bacleriuin calendulae 

 Takimoto, Ann. Phytopath. Soc. Japan, 

 5, 1936, 341; Phytomonas calendulae 

 Burkholder, in Manual, 5th ed., 1939, 

 201; Dowson, Trans. Brit. Mycol. Soc, 

 26, 1943, 9.) From Latin, calendae, 

 throughout the month; M.L. Calendula, 

 a generic name. 



Rods: 0.5 by 1 to 2 microns. Motile 

 with 1 to 3 polar flagella. Gram-nega- 

 tive. 



Green fluorescent pigment produced 

 in Uschinsky's and in Cohn's solutions. 



Gelatin: Not liquefied. 



Agar colonies: Circular, smooth, fiat, 

 dirty white. 



Broth: Turbid. 



Milk: No coagulation. 



Nitrites not produced from nitrates. 



Indole formed in small amount. 



No HiS produced. 



Acid but not gas from glucose and 

 glycerol. No acid from lactose or sucrose. 



Starch not hydrolyzed. 



Optimum temperature 27° to 30°C. 

 Maximum 37°C. Minimum 0° to 7°C. 



Habitat : Pathogenic on marigolds. 

 Calendula officinalis. 



116. Pseudomonas cichorii (Swingle) 

 Stapp. (Phytomonas cichorii Swingle, 

 Phytopath., 15, 1925, 730; Stapp, in Sor- 

 auer, Handbuchder Pflanzenkrankheiten, 

 2, 5 Auf., 1928, 291; Bacterium cichorii 

 Elliott, Bact. Plant Pathogens, 1930, 

 112.) From Gr. cichoria, chicory; M. 

 L. Cichoriiim, generic name. 



Probable synonyms : Pseudomonas en- 

 diviae Kotte, Phyt. Ztschr., 1, 1930, 609; 

 Phytomonas endiviae (Kotte) Clara, 

 Cornell Agr. Exp. Sta. Mem. 159, 1934, 

 26; and Bacterium formosaniim Okabe, 

 Jour. Soc. Trop. Agr., Formosa, 7, 1935, 

 65. 



Description from Clara (loc. cit.) which 

 is a description of a culture of Pseudo- 

 monas endiviae from Kotte. Swingle's 

 description is verj^ meager. 



Rods : 0.75 to 1.5 by 1.5 to 3.75 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: No liquefaction. 



Beef -extract agar colonies : Circular, 

 grayish-white with bluish tinge, raised 

 with slightly irregular edges. 



Broth : Turbid in 36 hours with a 

 smooth viscous pellicle. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S formed. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but not gas from glucose, galac- 

 tose, fructose, mannose, arabinose, xy- 

 lose, mannitol and glycerol. Alkaline 

 production from salts of acetic, citric, 

 lactic, malic, succinic and tartaric acids. 



