134 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Rhamnose, maltose, sucrose, lactose, raf- 

 finose and salicin not utilized. 



Starch not hydrolyzed. 



Slight growth in broth plus 6 per cent 

 NaCl. 



Optimum pH 6.8 to 7.1. Maximum 

 9.2 to 9.4. Minimum 5.0 to 5.3 (Kotte, 

 Phyt. Ztsch., 2, 1930, 453). 



Facultative anaerobe. 



Distinctive characters: Differs from 

 Pseudomonas inlyhi in that it does not 

 liquefy gelatin or reduce nitrates to ni- 

 trites. 



Source : Isolated from rot of French 

 endive, Cichorium intybus by Swingle 

 and by Okabe, and from C. endivia by 

 Kotte. 



Habitat : Pathogenic on endive, lettuce 

 and larkspur. 



117. Pseudomonas cissicola (Taki- 

 nioto) comb. nov. {Aplanobacter cissicola 

 Takimoto, Ann. Phytopath. Soc. Japan., 

 9, 1939, 43.) From Greek, cissus, ivy; 

 M. L. Cissus a generic name. 



Rods: 0.5 to 0.9 by 1.0 to 2.0 microns. 

 Non-motile. Capsules. Gram-negative. 



Green fluorescent pigment formed in 

 Uschinsky's solution. 



Gelatin: No liquefaction. 



Potato-extract agar colonies: Circular, 

 convex, smooth, and dirty white. 



Broth: Feeble clouding followed by 

 precipitation of pellicle and rim. 



Nitrites not produced from nitrates. 



Indole not formed. 



Hydrogen sulfide not produced. 



No acid nor gas from sucrose, glucose, 

 lactose and glycerol. 



Starch is not digested. 



Salt toleration is 3 per cent. 



Optimum temperature 30°C. Maxi- 

 mum 35°C. Minimum lO'C. Thermal 

 death point 49° to 50°C. 



Source : Isolated from black spots on 

 leaves of Japanese ivy, Cissus japonica in 

 Japan. 



Habitat : Pathogenic only on Cisstis 

 japonica. 



118. Pseudomonas nectarophila 

 (Doidge) Rosen and Bleeker. {Bac- 



terium nectarophila Doidge, Ann. Appl. 

 Biol., 4, 1917, 73; Phytomonas nectaro- 

 phila Bergey et al., Manual, 3rd ed., 

 1930, 262 ; Rosen and Bleeker, Jour. Agr. 

 Res., It6, 1933, 98.) From Gr. nectar, 

 nectar; philus, loving. 



Rods: 0.5 to 0.7 by 0.6 to 1.5 microns. 

 Motile with 1 to 5 polar flagella. Cap- 

 sules. Gram-negative. 



Green fluorescent pigment produced 

 in culture. 



Gelatin: No liquefaction. 



Nutrient agar colonies : Yellowish- 

 white, wet-shining, smooth, margins ir- 

 regular. 



Broth: Heavy turbidity in 24 hours. 

 Sediment. 



Milk: Cleared. 



Nitrites not produced from nitrates. 



Indole not formed. 



Acid from glucose and galactose. No 

 acid from sucrose. 



Starch hydrolysis feeble. 



Optimum temperature 25 to 30°C. 



Facultative anaerobe. 



Distinctive character: Differs from 

 Pseudomonas barkeri in that it does not 

 liquefy gelatin, nor produce indole. Pro- 

 duces capsules. 



Source : Isolated from blighted pear 

 blossoms in South Africa. 



Habitat: Pathogenic on pear blossoms. 



119. Pseudomonas viburni (Thorn- 

 berry and Anderson) Stapp. {Phytomonas 

 viburni Thornberry and Anderson, Phy- 

 topath., 21, 1931, 912; Stapp, Bot. Rev., 

 1, 1935, 407; Bacterium viburni Burgwitz, 

 Phytopathogenic Bacteria, Leningrad, 

 1935, 160.) From L. viburnum, the way- 

 faring tree; M. L. Viburnum, a generic 

 name. 



Rods: 0.5 to 1.0 by 1 to 2.0 microns. 

 Capsules present. Motile with 2 to 4 

 polar flagella. Gram-negative (Burk- 

 holder) ; not Gram-positive as stated. 



Green fluorescent pigment produced in 

 culture (Burkholder). 



Gelatin: No liquefaction. 



Glucose beef -extract colonies: Dull 

 gray, circular, edges entire. 



