FAMILY PSEUDOMONADACEAE 



135 



Broth: Turbid with pellicle. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H2S formed. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid from glucose and galactose, but 

 not sucrose (Burkholder). 



Starch : No hydrolysis. 



Slight growth in 3.5 per cent salt 

 (Burkholder). 



Optimum temperature 25°C. Mini- 

 mum 12°C. Maximum 35°C. 



Aerobic. 



Source : Isolated from angular leaf 

 spots and stem lesions on arrow-wood, 

 Viburnujn opulus, etc. 



Habitat : Pathogenic on Viburnum spp. 



120. Pseudomonas mori (Bo3^er and 

 Lambert) Stevens. {Bacterium vinri 

 Boyer and Lambert, Compt. rend. Acad. 

 Sci. Paris, 117, 1893, 342; Bacterium mori 

 Boyer and Lambert emend. Erw. Smith, 

 Science, 31, 1910, 792; Stevens, The Fungi 

 which Cause Plant Diseases, 1913, 30; 

 Bacillus mori Holland, Jour. Bact., 5, 

 1920, 222; Phytomonas mori Bergey et 

 al., Manual, 1st ed., 1923, 191.) From 

 Gr. moriun, mulberry; ]\L L. Moms, a 

 generic name. 



Synonyms: Elliott (Bact. Plant Path- 

 ogens, 1930, 166) lists Bacillus cuboni- 

 anus Macchiati, Staz. Sperim. Agr. Ital., 

 S3, 1892, 228 (Macchiati described the 

 disease due to Pseudomonas mori, but 

 gave an incorrect description of the patho- 

 gen) ; also Bacterium cubonianum Fer- 

 raris, Curiano le Plante, 6, 1928, 180 

 (Ferraris uses Macchiati 's name but the 

 description of Pseudomonas ynori). 



Description from Smith {loc. cit.). 



Rods: 0.9 to 1.3 by 1.8 to 4.5 microns. 

 Motile with a polar flagellum. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin : Not liquefied. 



Agar colonies : White, slow -growing, 

 smooth, flat, edges entire becoming un- 

 dulate. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole none or feeble production. 



Hydrogen sulfide not produced (Okabe, 

 Jour. Soc. Trop. Agr., 5, 1933, 166). 



No growth in broth plus 4 per cent salt 

 (Okabe, loc. cit.). 



No gas from carbohydrates. 



Temperature range 1°C to 35°C. 



Source: Smith isolated the pathogen 

 from blighted shoots of mulberry from 

 Georgia. Also received cultures from 

 Arkansas and the Pacific Coast. 



Habitat : Pathogenic on mulberry, 

 Morus. 



121. Pseudomonas stizolobii (Wolf) 

 comb. nov. {Aplanobacter stizolobii Wolf, 

 Phytopath., 10, 1920, 79; Bacterium stiz- 

 olobii McCulloch, Phytopath., 18, 1928, 

 460; Phytomonas stizolobii Bergey et al.. 

 Manual, 3rd ed., 1930, 280.) From Gr. 

 siizo, to prick; lobium, a little lobe; 

 Stizolobium, a generic name. 



Rods: 0.6 to 0.7 by 1.0 to 1.6 microns. 

 Non-motile (Wolf). Motile with a short 

 polar flagellum (McCulloch). Capsules. 

 Gram-negative. 



Gelatin : No liquefaction. 



Agar colonies : Circular, smooth, white, 

 raised and opaque. Margins entire to 

 slightly undulate. 



Broth: Slightly turbid throughout. No 

 pellicle or ring. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



No acid or gas in peptone broth plus 

 sugars. 



Starch not hydrolyzed. 



Optimum temperature 25° to 28°C. 



Distinctive characters : Differs from 

 Pseudomonas sojae (Pseudomonas gly- 

 cinea) in the smaller size of cell, and 

 absence of pellicle and dense clouding of 

 broth. The pathogen does not infect 

 soy bean. 



Source : Isolated from the leaf spot of 

 velvet bean. 



Habitat : Pathogenic on velvet bean, 

 Stizolobium deeringianium. 



