136 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



122. Pseudomonas viciae Uyeda. 

 (Uyeda in Takimoto, Jour. Plant Pro- 

 tect., Japan, 2, 1915, 845; Bacterium vi- 

 ciae Nakata, see Elliott, Bact. Plant 

 Pathogens, 1930, 259; Phytomonas viciae 

 Magrou, in Hauduroy et al.. Diet. d. Bact. 

 Path., Paris, 1937, 430.) From L. vicia, 

 vetch; M. L. Vicia, a generic name. 



Rods: 0.5 to 0.8 by 1.2 to 2.0 microns. 

 Motile with 2 to 4 polar flagella. Gram- 

 positive. 



Green fluorescent pigment produced in 

 culture. 



Gelatin colonies : Pale white, glisten- 

 ing, finally turning brown. No liquefac- 

 tion. 



Milk : Coagulates and clears. 



Nitrites not produced from nitrates. 



No H2S produced. 



Facultative anaerobe. 



Habitat : Pathogenic on the broad 

 bean {Vicia faba), the turnip (Brassica 

 rapa), the carrot (Daucus carota) and the 

 sweet potato {Ipomoea batatas). 



123. Pseudomonas alliicola Burk- 



holder. (Burkholder, Phytopath., 32, 

 1942, 146 ; Phytomonas alliicola Burk- 

 holder, ibid.) From L. allium, onion; 

 -cola, dweller. 



Rods: 0.7 to 1.4 by 1.05 to 2.8 microns. 

 Motile with 1 to several polar flagella, at 

 times bi -polar. Gram-negative. 



Gelatin : Liquefaction. 



Beef -extract peptone agar streaks : 

 Moderate in growth, white at first, later 

 dirty in appearance, edges wavy, consist- 

 ency viscid. Medium deep brown. 



Potato-glucose agar frequently be- 

 comes greenish. 



Broth: Turbid with light pellicle. 

 Brown. 



Milk : Cleared and litmus reduced . 

 Neutral. 



Nitrites produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Lipolytic action very strong. 



Acid but no gas from 1-arabinose, d- 

 xjdose, rhamnose, glucose, d-galactose, 

 fructose, d-lactose, maltose, sucrose, 



glycerol, mannitol and salicin. Alkali 

 from salts of acetic, citric, formic, hip- 

 puric, lactic, malic, succinic, tartaric 

 acids. 



Starch not hydrolysed. 



Slight growth in broth plus 4 per cent 

 salt. 



Aerobic. 



Optimum temperature 30°C. Maxi- 

 mum 41°C. Minimum 5°C. 



Source: Seven isolates from storage 

 rot of onion bulbs. 



Habitat: Pathogenic on onion bulbs, 

 Allium cepa. 



124. Pseudomonas gardeniae Burk- 

 holder and Pirone. (Burkholder and 

 Pirone, Phytopath., 31, 1941, 194; Phyto- 

 monas gardeniae Burkholder and Pirone, 

 ibid.) From M. L. Gardenia, a generic 

 name. 



Rods: 0.75 by 2.4 microns. Motile 

 with 1 to 2 polar flagella. Gram-nega- 

 tive. 



Gelatin: Liquefaction. 



Beef -extract peptone agar colonies: 

 Growth fair, white to dirty gray and vis- 

 cid. Medium becoming dark brown. 



Potato-glucose agar : No brown color. 



Broth: Turbid with pellicle. Dark 

 brown. 



Milk: Soft curd with pellicle. Clears 

 in zones. Litmus reduced. 



Nitrites produced from nitrates. 



Hydrogen sulfide not produced. 



Indole not formed. 



Acid from glucose, galactose, xylose, 

 rhamnose, sucrose, maltose, mannitol, 

 glycerol, and salicin. Alkali produced 

 from the salts of citric, malic, malonic, 

 succinic, tartaric and hyppuric acids. 

 Good growth in tyrosine and in aspara- 

 gine broth. 



Starch is not hydrolyzed. 



Aerobic. 



Source: Eight isolates from leaf spots 

 of gardenias in New Jersey. 



Habitat: Pathogenic on leaves of Gar- 

 denia jasminoides. 



125 Pseudomonas caryophylli Burk- 

 holder. (Burkholder, Phytopath., 31, 



