138 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Optimum temperature 35° to 37°C. 

 Maximum 41 °C. Minimum 10°C. 



Pathogenicity readily lost in culture. 



Source : Isolated from brown-rot of 

 solanaceous plants. 



Habitat : Soil pathogen in warm moist 

 climates attacking numerous species of 

 plants, especially potato, tobacco, and 

 tomato. . 



126a. Pseudomonas solanacearum 

 var. asiatica (Erw. Smith) Stapp. {Bac- 

 terium solanacearum var. asiaticum Erw. 

 Smith, Bact. in Relation to Plant Dis- 

 eases, 3, 1914, 282; Stapp, in Sorauer, 

 Handbuch der Pflanzenkrankheiten, 2, 

 5 Auf., 1928, 253; Phytomonas solana- 

 cearum var. asiatica Magrou, in Hau- 

 duroy et al., Diet. d. Bact. Path., Paris, 

 1937, 414.) From Gr. asiaticus, asiatic. 



Distinctive characters: Differs from 

 Pseudomonas solanacearum in that it 

 turns litmus milk and cream red. 



Source : Isolated by J. A. Honing from 

 diseased tobacco plants in Medan, Suma- 

 tra. 



127. Pseudomonas castaneae (Kawa- 

 mura) comb. nov. {Bacterium castaneae 

 Kawamura, Ann. Phytopath. Soc. Japan, 

 S, 1934, 15; Phytomonas castaneae Ma- 

 grou, in Hauduroy et al.. Diet. d. Bact. 

 Path., Paris, 1937, 343.) From M. L. 

 Castanea, a generic name. 



Rods: 0.8 to 1.2 by 1.0 to 1.8 microns. 

 Motile with 1 to 5 polar flagel la. Gram- 

 negative . 



Gelatin: Liquefied. 



Beef agar colonies : White, circular, 

 edges slightly undulate, viscid. 



Milk: No coagulation. Peptonized. 



Acid but not gas from glucose, sucrose 

 and glycerol. No acid from lactose. 



Optimum temperature 25° to 27°C. 

 Maximum 35°C. Minimum 3°C. 



Facultative anaerobe. 



Habitat : Causes water-soaked spotting 

 on leaves and shoots of chestnut, Cas- 

 tanea. 



128. Pseudomonas seminum Cayley. 

 (Cayley, Jour. Agr. Sci., 8, 1917, 461; 

 Bacterium seminum Stevenson, Foreign 



Plant Dis., U.S.D.A. Office of Sec'y-, 

 1926, 141 ; Phytomonas seminum Bergey 

 et al., Manual, 3rd ed., 1930, 272.) From 

 L. semen {seminus) seed. 



Rods : 1 .0 by 4.0 to 5.0 microns. Spore- 

 like bodies present. Capsules. Motile 

 with a single flagellum. Gram-positive. 



Gelatin: Rapid liquefaction. 



Agar colonies: White, more or less 

 circular, transparent, spreading. 



Broth: Turbid. Pellicle. 



Litmus milk: Milk becomes clear and 

 apricot color. 



Nitrites produced from nitrates. 



Acid but not gas from glucose and 

 sucrose. No acid from lactose. 



Starch : No hydrolysis. 



Optimum temperature 25°C. 



Facultative anaerobe. 



Source : Isolated from seeds, stems and 

 pods of diseased peas in England. 



Habitat : Pathogenic on peas. 



129. Pseudomonas passiflorae (Reid) 

 comb. nov. {Phytomonas passiflorae Reid, 

 New Zealand Jour. Sci. and Tech., 22, 

 1939, 264a.) From L. passio, passion; 

 flora, flower; M. L. Passiflora, a generic 

 name. 



Rods: 0.2 to 0.5 by 1.2 to 3.2 microns. 

 Motile with 1 to 5 polar flagella. Cap- 

 sules present. Gram-negative. 



Gelatin : Liquefied. 



Beef-peptone agar colonies : Small, flat, 

 smooth, dry, shining, translucent, gray- 

 ish and butyrous. 



Broth : Turbid in 4 days. Transient 

 pellicle. 



Milk: Slightly alkaline. No coagula- 

 tion nor clearing. 



Nitrites not produced from nitrates. 

 No growth on synthetic nitrate agar. 



Indole not formed. 



Hydrogen sulfide not formed. 



Acid reaction occurs in galactose, starch 

 and sucrose. No gas. 



Starch is not hydrolysed. 



Source : From diseased leaves and fruit 

 of the passion-fruit in New Zealand. 



Habitat : Pathogenic on Passiflora 

 edulis. 



