FAMILY PSEUDOMONADACEAE 



139 



130. Pseudomonas fabae (Yu) comb, 

 nov. {Phytomonas fabac Yu, Bull, of the 

 Chinese Bot. Soc, 2, 1936, 34.) From 

 Latin, faba bean. 



Rods: 0.8 to 1.1 by 1.1 to 2.8 microns. 

 Motile with 1 to 4 polar flagella. Gram- 

 negative. 



Gelatin: Liquefied. 



Nutrient agar colonies : Circular, en- 

 tire, viscid, glistening, raised, smooth to 

 wrinkled, white to salmon. Medium 

 amber. 



Broth: Turbid after 12 hours. Pellicle. 



Milk : Growth slow. Clears. 



Nitrites produced from nitrates. 



Indole production slight. 



Hydrogen sulfide not produced. 



Acid but not gas from glucose. No 

 acid nor gas developed from arabinose, 

 xylose, fructose, galactose, sucrose, 

 lactose, maltose, raffinose, dextrin, in- 

 ulin, mannitol or adonitol in a 1 per cent 

 Bacto-peptone broth. 



Starch: Verj^ weak diastatic action. 



Optimum temperature 35°C. ^laxi- 

 mum 37 to 38"C. Minimum 4°C. Ther- 

 mal death point 52 to 53°C. 



Aerobic. 



Growth retarded in 2 per cent salt. 

 Very slight growth in 3 per cent salt. 



Source: From diseased broad beans 

 at Nanking, China- 

 Habitat : Pathogenic on broad or Wind- 

 sor bean, Vicia faba. 



131. Pseudomonas astragali (Taki- 

 moto) comb. nov. {Bacterium astragali 

 Takimoto, Jour. Plant Protect., 17, 1930, 

 732; Phytomonas astragali Burkholder, 

 in Manual, 5th ed., 1939, 197.) From 

 M. L. Astragalus, a generic name. 



Description translated by Dr. K. 

 Togashi. 



Rods: 0.7 to 0.8 by 1.2 to 2.2 microns. 

 Motile, with 1 or 2 flagella. Gram- 

 negative. 



Gelatin: Liquefied. 



Agar plates: Growth somewhat slow, 

 colorless or grayish-white, entire margins, 

 more or less aqueous, butyrous. 



Uschinsky's medium : Growth vigorous, 

 turbid, not viscid, ring, and sediment. 



Milk: No coagulation of casein, slow 

 digestion. Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



Hydrogen sulfide produced in small 

 amount. 



No acid or gas from glucose, sucrose, 

 lactose and glycerol in broth. 



Starch not hydrolyzed. 



Temperature relations : Minimum be- 

 low 5° and maximum 33'^C. Thermal 

 death point 50° to 51 °C. 



Aerobic. 



Source : Species isolated from Astra- 

 galus sp. 



Habitat : Causes a black leaf -spot of 

 Astragahis sp. 



132. Pseudomonas colurnae (Thorn- 

 berry and Anderson) comb. nov. {Phyto- 

 monas colurnae Thornberry and Ander- 

 son, Phytopath., 27, 1937, 948.) From 

 the species, Corylus colurna. 



Rods: 0.8 to 1.0 by 1.0 to 1.8 microns. 

 Single, in pairs or chains. Capsules. 

 Motile with 1 to 2 polar flagella. Gram- 

 negative. 



Gelatin: Liquified. 



Glucose agar slants: Growth filiform, 

 raised, dull, smooth, opaque and viscid. 



Broth : Moderate turbidity. Ring. 



Milk : Peptonization complete with 

 acid production. No reduction of litmus 

 nor coagulation. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



No appreciable amount of gas from 

 xylose, glucose, sucrose or glycerol. 



Starch hydrolyzed. 



Optimum temperature 21°C. Mini- 

 mum 5°C. Maximum 35°C. Thermal 

 death point oO^C. 



Aerobic. 



Source : From leaves and young stems 

 of the Turkish hazelnut in Illinois. 



Habitat : Pathogenic on the Turkish 

 hazelnut, Coryltis colurna. 



