142 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Garden, 9, 1922, 383; Bacterium albo- 

 precipitans Elliott, Bact. Plant Path., 

 1930, 89; Phytomonas alboprecipitans 

 Bergey et al., Manual, 3rd ed., 1930, 

 277.) From Latin albus, white and 

 precipitans , precipitating, referring to 

 the white precipitate produced in cul- 

 ture. 



Rods: 0.6 by 1.8 microns, occurring 

 singly or in pairs. Capsules present. 

 Motile with a polar flagellum. Gram- 

 negative. 



Gelatin: Not liquefied. 



Nutrient agar colonies : White, circular, 

 raised, smooth, sticky, with margins 

 entire. Whitish discoloration of the 

 medium. 



Broth: Turbid in 24 hours. Heavy 

 sediment in old cultures. 



Milk : Becomes alkaline and slowly 

 clears. 



Nitrites produced from nitrates. 



Indole not produced. 



No HzS produced. 



Acid but not gas from glucose, fruc- 

 tose, glycerol and mannitol. No acid 

 from lactose, maltose or sucrose. 



Starch is hydrolyzed. 



Optimum temperature 30° to 35°C. 

 Maximum temperature 40 °C. Minimum 

 0°C. 



Aerobic. 



Distinctive characteristics : White pre- 

 cipitate in culture media. 



Source : Isolated a number of times 

 from foxtail grass. 



Habitat : Pathogenic on foxtail, Chaeto- 

 chloa lutescens and other grasses. 



141. Pseudomonas petasitis (Taki- 

 moto) comb. nov. {Bacterium petasitis 

 Takimoto, Ann. Phyt. Soc. Japan, 2, 

 1927, 55; Phytomonas petasitis Magrou, 

 in Hauduroy et al., Diet. d. Bact. Path., 

 Paris, 1937, 393.) From M. L. Petasites, 

 a generic name. 



Rods: 0.8 to 1.1 by 1.1 to 1.7 microns. 

 Motile with a polar flagellum. Gram- 

 negative. 



Gelatin: No liquefaction. 



Beef agar colonies : White, circular or 

 ameboid, butyrous. 



Broth: Strong turbidity. Pellicle. 



Milk: Coagulated in 30 days. 



Nitrites produced from nitrates with 

 gas formation. 



Indole not produced. 



No HoS produced. 



No evident acid in peptone broth but 

 gas from glucose, lactose and sucrose. 

 .\cid but not gas from glycerol. 



Weak growth in broth plus 6 percent 

 salt. 



Optimum temperature 27° to 30 °C. 

 Maximum 47°C. Minimum approxi- 

 mately 5°C. 



Source: Isolated from brown to black 

 lesions on Petasites japonicus in Japan. 



Habitat : Pathogenic on leaves of 

 Petasites japonicus. 



142. Pseudomonas lignicola Wester- 

 dijk and Buisman. (De lepenziekte, 

 Arnliem, 1929, 51.) From Latin, lignum, 

 wood; -cola, dweller. 



Rods: Single or short chains. Motile 

 with 1 to several polar flagella. Gram- 

 negative. 



Gelatin: No liquefaction. 



Malt agar streaks : Milk white with a 

 colorless edge. 



Broth : Turbid with light pellicle. 



Milk: No coagulation. No acid. 



Nitrites not produced from nitrates. 



Indole not formed. 



Starch hydrolysis slight. 



Optimum temperature ±25°C. 



Source : From vessels of elm wood 

 showing dark discoloration, in Holland. 



Habitat: Pathogenic in elm wood. 



143. Pseudomonas andropogoni (Erw. 

 Smith) Stapp. {Bacterium andropo- 

 goni Erw. Smith, Bacteria in Relation 

 to Plant Diseases, 2, 1911, 63; Elliott and 

 Smith, Jour. Agr. Res., 38, 1929, 4; Stapp, 

 in Sorauer, Handbuch der Pflanzen- 

 krankheiten, 2, 5 Auf., 1928, 27; Phyto- 

 monas andropogoni Bergey et al., 

 Manual, 3rd ed., 1930, 276.) From M. 



