144 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Facultative anaerobe. 



Source : Species first isolated from 

 millet, Panicum miliaceum. 



Habitat : Causes a leaf stripe of Pani- 

 cum miliaceum. 



146. Pseudomonas saliciperda Lindei- 

 jer. (Lindeijer, Inaug. Diss., Univ. 

 Amsterdam, 1932; Phytopath. Ztschr., 

 6, 1933, 373; Bacterium saliciperda 

 Burgwitz, Phytopathogenic Bacteria, 

 Leningrad, 1935, 106; Phytomonas salici- 

 perda Magrou, in Hauduroy et al., Diet, 

 d. Bact. Path., Paris, 1937, 408.) From L. 

 salix (salicis), willow; perdo, to destroy. 



Rods: 1.2 to 2.1 microns in length. 

 Motile with a polar fiagellum. Gram- 

 negative. 



Gelatin: No liquefaction. 



Beef wort agar colonies: Gray -white. 



Milk: No acid nor coagulation. 



Nitrites produced (small amount) 

 from nitrates. 



Indole formation slight. 



No gas from carbohydrates. 



Starch not hydrolyzed. 



Facultative anaerobe. 



Source: Isolated from wilted branches 

 of willow and pathogenicity proved. 



Habitat : Pathogenic on willow, Salix 

 spp. 



147. Pseudomonas eriobotryae (Taki- 

 moto) Dowson. (Bacterium eriobotryae 

 Takimoto, Jour. Plant Protect., 18, 1931, 

 354; Phytomonas eriobotryae Burkholder, 

 in Manual, 5th ed., 1939, 205; Dowson, 

 Trans. Brit. Mycol. Soc, 26, 1943, 10.) 

 From M. L. Eriobotrya, a generic name. 



Translated by Dr. K. Togashi. 



Rods: 0.7 to 0.9 by 2.2 to 3.0 microns. 

 Motile, w^ith 1 or 2 flagella. Gram- 

 negative. 



Gelatin: Not liquefied. 



Agar-plates : Colonies appear after 3 

 days, white or hyaline, butyrous, margins 

 entire. 



Broth: Moderately turbid, pellicle 

 powdery, ring formed. 



Milk : No coagulation, peptonized 

 slowly. Alkaline. 



Nitrites not produced from nitrates. 



Indole not formed. 



No H^S produced. 



No acid or gas from glucose, sucrose, 

 lactose and glycerol in broth. 



Starch not hydrolyzed. 



Temperature relations : Minimum 

 below 4°C, optimum 25° to 26°C, and 

 maximum 32°C. Thermal death point 

 51°C. 



Aerobic. 



Source : Species isolated from loquat, 

 Eriobotrya japonica. 



Habitat : Causes a bud rot of Eriobotrya 

 japonica. 



148. Pseudomonas wieringae (Elliott) 

 comb. nov. {Phytomonas betac Wieringa, 

 Nederl. Tijdschr. Hyg., Microbiol, en 

 Serol., Leiden, 2, 1927, 148; Bacterium 

 wieringae Elliott, Man. Bact. Plant 

 Pathogens, 1930, 264; Phytomonas wier- 

 ingae Burkholder, in Manual, 5th ed., 

 1939, 206.) Named for K. L. Wieringa, 

 plant pathologist of Holland. 



Because Bacterium betae Chester (Ann. 

 Kept. Del. Col. Agr. Exp. Sta., 9, 1897, 

 53) may be a pseudomonad, the species 

 name proposed by Elliott has been 

 retained. 



Description from Elliott (loc. cit.). 



Rods : 0.5 to 2.0 microns. Motile with 

 1 to 5 polar flagella. Gram-negative. 



Beef-agar colonies: Smooth, round, 

 white to grayish, fluorescent. 



Milk: Cleared in 5 days. Not co- 

 agulated. 



Nitrites not produced from nitrates. 



No gas from sugars. 



Optimum temperature 28° to 30 °C. 

 Maximum 37°C. Minimum 4°C. 



Source : Isolated from vascular rot of 

 beets in Holland. 



Habitat : Pathogenic on beets, Beta 

 vulgaris. 



