154 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Optimum pH 6.5. Maximum 9.0 to 

 9.5. Minimum 4.5 to 4.8. 



Tolerates salt up to 9 per cent. 



Aerobic. 



Source : Isolated from galls on sugar 

 beets collected in Colorado, Kansas, 

 and Virginia. 



Habitat : Produces gall on sugar beets 

 and on garden beets. 



Note : It is doubtful whether this 

 species belongs in this genus. 



5. Xanthomonas lactucae-scariolae 



(Thornberry and Anderson) comb. nov. 

 {Phytomonas lactucae-scariolae Thorn- 

 berry and Anderson, Phytopath., 27, 

 1937, 109.) From Lactuca scariola, the 

 host. 



Rods: 0.5 to 1.0 by 1.0 to 1.5 microns. 

 Motile with 1 or 2 polar flagella. Chains 

 present. Capsules. Gram-negative. 



Gelatin : Slow liquefaction. 



Glucose agar colonies : Round, entire, 

 finely granular, amber yellow. 



Broth: Turbid. No pellicle. A yel- 

 low rim. 



Milk: Slight acid, and peptonization. 



Nitrites are produced from nitrates. 



Hydrogen sulfide not formed. 



No gas from carbon sources. 



Starch: Slight diastatic activity. 



Optimum temperature 25 °C. Maxi- 

 mum 35°C. Minimum 7°C. 



Aerobic. 



Source : Isolated from necrotic lesions 

 on wild lettuce. 



Habitat : Pathogenic on wild lettuce, 

 Lactuca scariola, but not on cultivated 

 lettuce, Lactuca sativa. 



6. Xanthomonas rubrilineans (Lee et 

 al. ) Starr and Burkholder. {Phytomonas 

 rubrilineans Lee, Purdy, Barnum and 

 Martin, Haw^aiian Sugar Planters' Assoc. 

 Bull., 1925, 25; Pseudomonas rubrilineans 

 Stapp, in Sorauer, Handb. d. Pflanzen- 

 krank., 2, 5 Aufi., 1928, 35; Bacterium 

 rubrilineans Elliott, Man. Bact. Plant 

 Path., 1930, 195; Starr and Burkholder, 

 Phytopath., 32, 1942, 600.) From L. 

 ruber, rsd; lineans, striping. 



Rods: 0.7 by 1.67 microns. Motile 

 with 1 or seldom more polar flagella. 

 Gram-negative. 



Gelatin: Liquefaction. 



Agar (Beef -extract + glucose) col- 

 onies: Small, smooth, glistening, buff to 

 yellow. 



Broth: Turbid with pellicle. Sedi- 

 ment. 



Milk : Casein precipitated and digested. 



Nitrites are produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not formed. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., S2, 1942, 600). 



Acid from glucose, fructose, arabinose, 

 xylose, lactose, sucrose, raffinose and 

 mannitol. 



Starch: Slight hydrolysis. 



Growth range, pH 5.4 to pH 7.3. 



Facultative anaerobe. 



Source : Description from 3 cultures 

 isolated from the red stripe lesions in 

 sugar cane. 



Habitat : Pathogenic on sugar cane. 



7. Xanthomonas barbareae Burkholdei , 

 (Burkholder, Phytopath., 31, 1941, 348- 

 Phytomonas barbareae Burkholder, ibid.) 

 From M. L. Barbarea, a generic name. 



Rods : 0.4 to 0.95 by 1.0 to 3.15 microns. 

 Motile with a single polar flagellum. 

 Gram-negative. 



Gelatin : Liquefaction. 



Beef -extract peptone colonies : Cir- 

 cular, yellow, smooth, butyrous, growth 

 moderate. 



Potato glucose agar : Growth abun- 

 dant, pale yellow. Mucoid. 



Broth : Turbid, yellow granular ring. 



Milk: Soft curd, with clearing and 

 production of tyrosine crystals. Litmus 

 reduced. 



Nitrates utilized but no nitrites formed. 

 Asparagine and nitrites not utilized. 



Hydrogen sulfide produced. 



Indole not formed. 



Lipolytic (Starr and Burkholder, loc. 

 cit.). 



Acid from glucose, galactose, xylose, 

 maltose, sucrose, and glycerol. Alkali 



