160 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Optimum temperature 25° to 30 °C. 

 Maximum 36° to 38 °C. (Elliott, loc. cit.). 



Source : Isolated from angular leaf spot 

 of cotton. 



Habitat : Pathogenic on cotton where - 

 ever it is grown, causing a leaf spot, a 

 stem lesion and a boll lesion. 



19. Xanthomonas pelargonii (Brown) 

 Starr and Burkholder. {Bacterium pe- 

 largoni Brown, Jour. Agr. Res., 28, 1923, 

 372; Pseudomonas -pelargoni Stapp, in 

 Sorauer, Handb. d. Pflanzenkrank, 2, 5 

 Aufl., 1928, 181; Phytonionas pelargonii 

 Bergey et al., Manual, 3rd ed., 1930, 

 250; Starr and Burkholder, Phytopath., 

 32, 1942, 600.) P'rom Greek, pelargus, 

 the stork; M. L. Pelargonium, a generic 

 name for the stork's bill geranium. 



Rods : .67 by 1 .02 mi crons . Capsules . 

 Motile with a polar flagellum. Gram- 

 negative. 



Gelatin: Slow liquefaction. 



Beef -agar colonies : Cream -colored, 

 glistening, round, with delicate internal 

 markings. 



Broth: Turbid in 24 hours. Incom- 

 plete pellicle. 



Milk: Alkaline. Clearing in bands. 



Nitrites not produced from nitrates. 



Indole formation slight. 



Hydrogen sulfide produced. 



Lipolytic (Starr and Burkholder, Phy- 

 topath., S;2, 1942, 600). 



Slight acid but not gas from glucose, 

 sucrose and glycerol. 



Starch hydrolysis feebly positive. 



Optimum temperature 27 °C. Maxi- 

 mum 35 °C. 



No growth in broth plus 3.5 per cent 

 salt. 



Aerobic. 



Source: Isolated from spots on leaves 

 of Pelargonium from District of Colum- 

 bia, Maryland and New Jersey. 



Habitat: Pathogenic on Pelargonium 

 spp. and Geranium spp. 



20. Xanthomonas phaseoli (Erw. 

 Smith) Dowson. {Bacillus phaseoli Erw. 

 Smith, Bot. Gaz., 2^, 1897, 192; A. A. A. 



S. Proc, JiB, 1898, 288; Pseudomonas 

 phaseoli Erw. Smith, U. S. Dept. Agr., 

 Div. Veg. Phys. and Path., Bui. 28, 1901, 

 1 ; Bacterium phaseoli Erw. Smith, 

 Bact. in Rel. to Plant Dis., 1, 1905, 72; 

 Phytonionas phaseoli Bergey et al.. 

 Manual, 1st ed., 1923, 177; Dowson, 

 Cent. f. Bakt., II Abt., 100, 1939, 190.) 

 From Gr. phaseolus, the bean; M. L. 

 Phaseolus, a generic name. 



Description from Burkholder, Cornell 

 Agr. Exp. Sta. Mem. 127, 1930, 18; 

 and Phytopath., 22, 1932, 609. 



Rods: 0.87 by 1.9 microns. Motile 

 with a polar flagellum. Gram-negative. 



Gelatin : Liquefaction. 



Beef -extract agar colonies: Circular, 

 amber yellow, smooth, butyrous, edges 

 entire. 



Broth : Turbid in 24 hours. Yellow 

 ring. 



Milk : Casein precipitated and digested. 

 Alkaline. Tyrosine crystals formed. 



Nitrites not produced from nitrates. 



Indole not formed. 



Hydrogen sulfide produced. 



Lipolytic (Starr and Burkholder, Phy- 

 topath., 32, 1942,600). 



Acid but not gas from glucose, galac- 

 tose, fructose, arabinose, xylose, maltose, 

 lactose, sucrose, raffinose and glycerol. 

 Alkaline reaction from salts of acetic, 

 malic, citric and succinic acids. Man- 

 nitol, dulcitol, salicin and formic and 

 tartaric acids not fermented. 



Starch is hydrolyzed. 



Aerobic. 



Very slight growth in beef broth plus 

 4 per cent salt (Hedges, Jour. Agr. Res., 

 ^,9,1924,243). 



Distinctive character: Similar in cul- 

 ture to Xanthomonas campestris, X. 

 juglandis, X. vesicatoria, etc., but they 

 do not cross infect . 



Habitat: Pathogenic on the bean 

 {Phaseolus vulgaris), the hyacinth bean 

 {Dolichos lablab), the lupine {Lupinus 

 polyphillus) , etc. Not pathogenic on 

 the soy bean {Glycine sp.), nor cowpea 

 {Vigna sp.). 



