174 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Rods: 0.5 to 0.6 by 0.4 to 1.4 microns. 

 Motile, possessing a single or occasionally 

 two or more very long (20 microns) polar 

 flagella. Gram-negative. 



Gelatin colonies: Circular, yellow, 

 granular. 



Gelatin stab : Pale-yellow surface 

 growth. Brownish yellow under surface 

 colonies. Saccate liquefaction. 



Agar slant : Dark yellow, glistening, 

 with dark yellow sediment in water of 

 condensation. Pigment not water-sol- 

 uble. 



Broth: Turbid. 



Litmus milk: Slightly acid. Litmus 

 reduced. 



Potato : Grayish yellow to brownish 

 growth. 



Indole formed. 



Nitrites are produced from nitrates. 



Acid formed in glucose. 



Starch hydrolyzed. 



Blood serum not liquefied. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Source: Air contamination. 



4. Pseudomonas caudata (Wright) 

 Conn. {Bacillus caudatus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 444; 

 Bacterium caudatus Chester, Annual 

 Rept. Del. Col. Agr. Exp. Sta., 9, 1897, 

 107; Conn, Jour. Agr. Res., 16, 1919, 313; 

 Flavobacterium caudatum Bergey et al., 

 Manual, 1st ed., 1923, 109.) From 

 Latin, cauda, tail. 



Rods : Long, granular, slender, occurring 

 singly, in pairs and in chains. Appear 

 like cocci in old cultures. Motile, pos- 

 sessing a polar flagellum (Conn). Gram- 

 negative. 



Gelatin colonies: Yellow, translucent, 

 smooth, undulate. 



Gelatin stab: Villous growth in stab. 

 Crateriform liquefaction. 



Agar slant: Yellow to orange, glisten- 

 ing, translucent, slightly spreading. 

 May lose power to form pigment. 



Broth: Turbid, with yellow sediment. 



Litmus milk: Unchanged. 



Potato: Dark yellow, raised, rough, 

 spreading. 



Indole not formed. 



Nitrites and ammonia produced from 

 nitrates. 

 Ammonia produced from peptone. 

 Starch is digested. 

 Aerobic, facultative. 

 Optimum temperature 25°C. 

 Habitat : Water. 



5. Pseudomonas perlurida Kellerman 

 et al. (Kellerman, McBeth, Scales and 

 Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 516; also McBeth, Soil Sci., 1, 1916, 472; 

 Ccllulomonas perlurida Bergey et al., 

 Manual, 1st ed., 1923, 163.) 



Rods: 0.4 by 1.0 micron. Motile with 

 one to three polar flagella. Gram- 

 negative. 



Gelatin stab: Liquefaction. 



Agar slant : Moderate, flat, faint yellow 

 growth. 



Broth: Turbid in 5 days. 



Litmus milk : Acid. Peptonization 

 after 16 days. 



Potato : Scant yellow growth with 

 bleaching along line of growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia is produced. 



Acid from ghicose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source : Soil from Virginia, Louisiana 

 and Missouri . 



Habitat: Soil. 



5a. Pseudomonas perlurida var. vir- 

 giniana Kellerman et al. {loc. cit.). 

 Does not grow on potato and liquefies 

 gelatin rapidly. 



Source : Soil from Virginia. 



6. Pseudomonas iridescens Stanier. 

 (Jour. Bact., 43, 1941, 542.) From Latin, 

 iridescent. 



Rods: 0.2 to 0.3 by 1.5 to 7.0 microns, 

 average length 5.0 to 6.0 microns, occur- 

 ring singly. Non-motile. Gram-nega- 

 tive. 



