FAMILY PSEUDOMOXADACEAE 



175 



Sea water gelatin stab : Filiform growth. 

 Liquefaction by some strains. 



Sea water agar colonies: Concave, 2 to 

 3 mm in diameter, smooth, glistening, 

 translucent, pale yellow, edge irregular. 

 After 2 to 3 days a marked iridescence. 

 Later colonies rough, opaque, bright 

 yellow, sunken central portion with trans- 

 lucent periphery. 



Sea water agar slant : Growth spreading, 

 smooth, glistening, translucent, pale 

 yellow, iridescent, butyrous. 



Sea water broth: Turbid, light yellow, 

 granular pellicle. 



Indole not formed. 



Nitrites not produced from nitrates. 



Hydrogen sulfide not produced. 



Catalase positive. 



Urease negative. 



Acid from xylose, glucose, galactose, 

 /actose, maltose, sucrose and cellobiose. 

 No acid from arabinose. Starch and 

 cellulose are attacked. 



Aerobic. 



Optimum temperature 23°C. Mini- 

 mum o°C. Maximum 30°C. 



Salt range : 0.25 to 6.0 per cent. Opti- 

 mum 1.0 to 4.0 per cent. 



Source : Sea water. 



Habitat : Common along the coast of 

 the North Pacific. 



7. Pseudomonas turcosa (Zimmer- 

 mann) Migula. {Bacillus turcosa Zim- 

 mermann, Bakt. unserer Trink- und 

 Nutzwasser, Chemnitz, 2, 1894, 32; 

 Migula, Syst. d. Bakt. 2, 1900, 937; Flavo- 

 bacteriiim turcosuvi Bergej^ et al., [Man- 

 ual, 1st ed., 1923, 111.) From :\r. L. 

 turcois, turquoise. 



Rods: 0.5 by 1.05 to 1.82 microns, oc- 

 curring singly. A short polar fiagellum 

 (Migula). Gram-negative. 



Gelatin colonies: Small, translucent, 

 yellow. 



Gelatin stab: Small, yellow, convex 

 surface growth, with slight brownish 

 tint. Liquefaction, with grayish to 

 greenish color in liquefied portion. 



Agar slant: Abundant, glistening, 

 greenish to sulfur yellow streak. 



Broth: Slightly turbid with yellow 

 sediment. 



Litmus milk: No coagulation. Yellow 

 sediment. 



Potato : Clear chromium yellow growth 

 over entire surface. 



Indole is not formed. 



Nitrites not produced from nitrates. 



Acid from glucose. Slight action on 

 sucrose. 



Aerobic, facultative. 



Optimum temperature 30''C. 



Source : Isolated by Tataroff from a 

 well in Dorpat (Die Dorpaten Wasser- 

 bakterien, Inaug. Diss., 1891, 52, No. 24). 



Habitat : Water, sea water. 



8. Pseudomonas ochracea (Zimmer- 

 mann) Chester. {Bacillus ochraceus 

 Zimmermann, Bakt. unserer Trink- und 

 Nutzwasser, Chemnitz, 1, 1890, 60; 

 Chester, Determinative Bacteriology, 

 1901, 316; Flavohacteritnn ochraceum Ber- 

 gey et al., Manual, 1st ed., 1923, 110; 

 Chromobacterium ochraceum Topley and 

 Wilson, Princ. Bact. and Immun., 1, 

 1931, 405.) From Greek, ochros, pale 

 yellow. 



Rods : 0.7 to 0.8 by 1.2 to 4.5 microns, 

 occurring in pairs and longer chains. 

 Slow undulatory motion (Zimmermann). 

 Polar flagella (Lehmann and Neumann, 

 Bakt.Diag.,lAufl.,^, 1896,255). Gram- 

 negative. 



Gelatin colonies : Pale yellow to golden, 

 ochre yellow, slightly raised, with slightly 

 fringed margin, granular. 



Gelatin stab : Yellowish to yellow-gray 

 surface growth. Infundibuliform lique- 

 faction. Pale yellow to ochre yellow 

 sediment. 



Agar colonies: Thin, flat, j^ellowish, 

 smooth. 



Agar slant: Thin, yellowish-gray to 

 ochraceous growth. 



Broth: Slightly turbid, with pale yel- 

 low sediment. 



Litmus milk: Medium becomes slimy; 

 alkaline. 



Potato : Ochre-yellow streak. 



Indole is formed. 



