176 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Nitrites not produced from nitrates. 

 Hydrogen sulfide is formed. 

 Aerobic, facultative. 

 Optimum temperature 35°C. 

 Source: Chemnitz tap water. 

 Habitat: Water. 



9. Pseudomonas cerevisiae Fuhr- 

 mann. (Fuhrmann, Cent. f. Bakt., II 

 Abt., 16, 1906, 309; Flavohacterium 

 cerevisiae Bergey et al., Manual, 1st ed., 

 1923, 111.) From Latin, cerevisia, beer. 



Rods: Straight and slightly curved, 

 0.6 by 1.5 to 2.0 microns, occurring singly 

 and in chains. Motile, possessing tuft, 

 four to six polar fiagella. Gram-negative . 



Gelatin colonies: Circular, white, 

 slightly contoured, becoming brownish- 

 yellow. 



Gelatin stab: Slight yellowish growth 

 in stab. No liquefaction. 



Agar colonies: Thin, spreading, con- 

 toured. 



Agar slant : Moist, glistening, thin, pale 

 yellow, spreading, contoured. 



Litmus milk: Slow coagulation. 



Potato : Yellowish-brown, spreading 

 growth. 



Indole not formed. 



Nitrites produced from nitrates. 



No gas from glucose . 



Aerobic, facultative. 



Optimum temperature 30°C. 



Source: Isolated from beer. 



Habitat: Unknown. 



10. Pseudomonas arguta McBeth. 

 (McBeth, Soil Science, 1, 1916, 465; 

 Cellulomonas arguata (sic) Bergey et al., 

 Manual, 1st ed., 1923, 164.) From Latin, 

 arguo, to show. 



Rods: 0.3 by 0.8 micron. Motile with 

 one or two polar fiagella. Gram-negative. 



Gelatin stab: Moderate, yellowish 

 growth. No liquefaction in 30 days. 



Agar colonies: Circular, slightly con- 

 vex, soft, grayish-white, granular, entire. 



Agar slant: Scant, grayish-white 

 growth. 



Potato agar slant: Moderate, yellow- 

 ish, glistening. 



Broth: Turbid. 



Ammonia cellulose agar: Enzymatic 

 zone 2 to 3 mm in 30 days. 



Filter paper broth : Paper is reduced 

 to loose fiocculent mass which disinte- 

 grates very readily on slight agitation. 

 More rapid decomposition when the 

 broth contains ammonium sulfate, po- 

 tassium nitrate, peptone or casein as 

 sources of nitrogen. 



Litmus milk : Acid, not digested. 



Potato: No growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 starch. No acid from glycerol, manni- 

 tol or sucrose. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Isolated twice from California 

 soils. 



Habitat: Soil. 



1 1 . Pseudomonas subcreta McBeth 

 and Scales. (McBeth and Scales, Bur. 

 Plant Industry, U. S. Dept. Agr., Bui. 

 266, 1913, 37; Cellulomonas subcreta 

 Bergey et al., Manual, 1st ed., 1923, 164.) 

 From Latin, sub, under, imperfect; creta 

 chalk. 



Rods : 0.3 by 1.4 microns. Motile with 

 one to five polar fiagella. Gram-negative. 



Gelatin stab : Filiform growth, no lique- 

 faction. 



Cellulose agar: No surface growth. 

 Moderate, generally faint yellow growth 

 in medium, area of growth sunken. 



Agar slant: Glistening, smooth, moisl., 

 vitreous to faint yellow. 



Starch agar : Enzymatic zone 2 to 4 mm. 



Broth : No growth. 



Litmus milk : No growth. 



Potato : Growth scanty, concave due to 

 slight liquefaction, white to faint yellow. 

 Bleached around growth. 



Indole not formed. 



Tr^ce of nitrites produced from ni- 

 trates. 



Ammonia not produced. 



Acid from glucose, lactose, maltose, 



