FAMILY PSEUDOMONADACEAE 



177 



sucrose and starch. No acid from glyc- 

 erol or mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Habitat : Soil. 



12. Pseudomonas pictorum Gray 

 and Thornton. (Gray and Thornton, 

 Cent. f. Bakt., II Abt., 73, 1928, 89; 

 Achromobacter pictorum Bergey et al., 

 Manual, 3rd ed., 1930, 217.) From 

 Latin, picti, the Pict.s of Eastern Scot- 

 land. 



Rods: 0.5 to 0.8 by 1.5 to 5.0 microns. 

 Motile usually with a single polar flagel- 

 lum. Gram-negative. 



Gelatin colonies : Circular, greenish- 

 yellow, convex, smooth, glistening, en- 

 tire. 



Gelatin stab: Xo liquefaction. 



Agar colonies : Circular, j^ellow, convex, 

 smooth, glistening, entire. 



Agar slant: Filiform, yellow, convex, 

 smooth, glistening, entire. 



Broth: Turbid. 



Nitrites produced from nitrates. 



Starch not hydrolj^zed. 



Acid from glucose and maltose. 



Attacks phenol. 



Aerobic, facultative. 



Optimum temperature 25''C. 



Source: One culture from soil. 



Habitat : Soil. 



13. Pseudomonas lacunogenes Gor- 

 esline. (Jour. Bact., 26, 1033, 447.) 

 From Latin lacuno, dimple and gcnero, 

 to produce. 



Short rods: 0.2 to 0.3 by 1.0 to 1.2 

 microns, with pointed ends, occurring 

 singly or in pairs. Motile with a single 

 polar flagellum from 2 to 15 microns in 

 length. Gram-negative. 



Plain gelatin stab : No growth. 



Nutrient gelatin stab : Growth brown- 

 ish-yellow, half-way down stab, heavier 

 at surface. No liquefaction. 



Nutrient agar colonies: Small, yellow; 

 surface of the agar pitted or dimpled. 

 After 5 days colonies 5 to 7 mm in diam- 



eter, orange-yellow, slightly raised, sur- 

 rounded by a depression. 



Nutrient agar slant: Growth heavy, 

 light orange-yellow ; consistency of warm 

 butter; edge entire, slightly raised. 

 Shallow depression formed on each side 

 of streak . Agar softened beneath growth . 

 Nutrient broth: Turbid in 48 hours. 

 Light orange-yellow pellicle ; considerable 

 viscous sediment. 



Litmus milk: Alkaline; butter-colored 

 pellicle. Reduction in bottom of tube 

 after 10 days. No curd. No digestion. 

 Potato: Growth moderate, orange-yel- 

 low, smooth. No darkening. 

 Indole not formed. 

 Nitrites not produced from nitrates. 

 Starch agar plates not hydrolyzed. 

 Utilizes arabinose, galactose, lactose, 

 fructose, maltose, melezitose, raffinose, 

 starch, xylose, glucose, mannose, su- 

 crose, pectin, rhamnose, salicin and 

 dextrin. No growth in dulcitol, erythri- 

 tol, glycerol, sorbitol, mannitol or inulin. 

 Limits of pH : 5.4 to 10.0.' 

 Temperature relations : Optimum 28''C. 

 Good growth at 25°C. Moderate growth 

 at 20° and at 37°C. No growth at 10° 

 and at 42°C. 

 Facultative anaerobe. 

 Distinctive characters: Softens agar; 

 considerable change in viscosity of agar 

 due to this digestion; utilization of 

 ammonium sulfate as nitrogen source. 



Source : Three cultures isolated from 

 an experimental trickling filter receiving 

 creamery wastes. 



Habitat : Probably widely distributed 

 in nature. 



14. Pseudomonas segnis Goresline. 

 (Jour. Bact., 26, 1933, 452.) From Latin 

 segne, non-energetic. 



Short rods: 0.2 to 0.3 by 1.0 to 1.2 mi- 

 crons, with pointed ends, occurring singly 

 or in pairs. Motile with a single polar 

 flagellum. Gram-negative. 



Plain gelatin stab: No growth. 



Nutrient gelatin stab: Growth yellow, 

 half-way down stab, best at surface. 

 No liquefaction. 



