178 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Nutrient agar colonies: Very small, 

 light yellow; surface pitted. After 5 

 days colonies 5 mm in diameter. 



Nutrient agar slant : Grovv'th heavy, 

 orange-yellow, consistency of warm but- 

 ter; edge entire, slightly raised; slight 

 depression formed on each side of growth. 

 Agar softened beneath growth. 



Nutrient broth: Turbid in 48 hours. 

 No pellicle or surface growth. Moderate 

 amount of sediment. Old cultures with a 

 yellow ring at surface and occasionally a 

 loose membrane. 



Litmus milk : Slightly alkaline after 10 

 days. No reduction. No surface growth. 



Potato: Scant yellow-orange growth. 

 No darkening. 



Indole not formed. 



Nitrites not produced from nitrates. 



No HoS produced. 



Starch not hydrolyzed. 



Utilizes arabinose, glucose, galactose, 

 lactose, fructose, maltose, mannose, 

 xylose, sucrose, melezitose and raffinose. 



Limits of pH : 5.8 to 9.0. 



Temperature relations : Optimum 28°C. 

 Good growth at 25°C. Moderate growth 

 at 20° and at 37°C. No growth at 10° 

 and at 42°C. 



Facultative anaerobe. 



Distinctive characters: Softens agar; 

 considerable change in viscosity of agar 

 due to this digestion. 



Source : Isolated from an experimental 

 trickling filter receiving creamery wastes. 



Habitat : Probably widely distributed 

 in nature. 



15. Pseudomonas lemonnieri (Lasseur) 

 comb. nov. (Bacillus lemonnieri Lasseur, 

 Compt. rend. Soc. Biol. Paris, 74, 1913, 

 47; Bui. de la Soc. des Sci. de Nancy, 

 1924; Flavobacicrium lasseuri Bergey et 

 al.. Manual, 3rd ed., 1930, 144.) Named 

 for Prof. G. le Monnier, a French 

 scientist. 



Rods: 0.5 to 0.7 by 1.0 to 2.0 microns, 

 occurring singly and in pairs. Motile 



with a single polar flagellum. Gram- 

 negative. 



Gelatin colonies (glucose) : Circular 

 with blue center, a granular, yellow zone 

 and a peripheral blue zone. Rapid 

 liquefaction with blue crystals. 



Gelatin stab : Liquefied. 



Agar colonies: Circular, yellowish, 

 lobate margin. 



Agar slant : Yellowish streak, smooth, 

 glistening. 



Broth: Turbid with thin pellicle. 



Litmus milk: After 48 hours the sur- 

 face of the milk becomes yellow to cream 

 color turning blue. A soft coagulum is 

 formed. 



Potato : Raised growth, Prussian blue 

 in color, with variations. 



Indole is not formed. 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 22° to 25°C. 



Habitat : Water. 



Appendix II:* The following inade- 

 quately described species may belong 

 to the genus Xanthomonas. 



Bacterium citri deliciosae Passalacqua. 

 (Rev. Pat. Veg., 24, 1934, 27.) Isolated 

 from Citrvs sp. 



Bacterium malvacearum var. harbadense 

 Evelyn. (Ann. Rept. Agric. Barbados 

 for 1926-27, 1928, 15.) Isolated from 

 cotton. 



Pseudomonas amaranti (sic) Smith. (U. 

 S. Dept. Agr., Div. Veg. Phys. and Path. 

 Bull., 28, 1901, 153; Bacterium amaranthi 

 Smith, Bact. in Relation to Plant Dis., 

 3, 1914, 148; Phytomonas amaranthi 

 Bergey et al., Manual, 1st ed., 1923, 186.) 

 Isolated from diseased amaranthus. 

 Growth in culture similar to Xantho- 

 monas campestris and Xanthomonas hya- 

 cinthi. 



Pseudomonas alutacea Migula. (Led- 

 crgelber Bacillus, Tataroff, Die Dorpater 

 Wasserbakterien, Inaug. Diss., Dorpat, 



* Prepared by Prof. Robert S. Breed, New York State Experiment Station, Geneva, 

 New York, July, 1943. 



