190 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Gelatin colonies: Circular, dry, light 

 yellow or colorless. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, opaque, pig- 

 ment bright red, yellow, light gray or 

 colorless. 



Amine agar colonies : Circular, white to 

 dark yellow. 



See table below for list of organic sub- 

 stances utilized. 



2. ProtaminobacterrubrumdenDooren 

 de Jong. (Thesis, Rotterdam, 1926, 

 159; also see Cent. f. Bakt., II Abt., 

 71, 1927, 218.) From Latin, ruber, red. 



Rods : Motile with single polar flagel- 

 lum (Weaver, Samuels and Sherago, 

 Jour. Bact., S5, 1938, 59). Gram-nega- 

 tive. 



Gelatin colonies : Circular, red, dry. 



Gelatin stab : No liquefaction. 



Table I. — Organic Substances Utilized as a Source of Carbon by Varieties of 

 Protaminobacter alboflavum 



ORGANIC ACIDS 



Acetic 



Valerianic 



a-crotonic 



Undecyclic 



Lactic 



|8-oxybutyric 



Succinic 



Formic 



Glutaric 



Adipic 



Fumaric 



Malic 



Tartaric 



Citric 



/S-phenylpropi- 



onic 



Quinic 



+ 



+ + 



AMINO COxMPOUNDS a 



a-alanin 



a - aminocapronic 



acid 



Leucin 



Propionamid . . 

 Capronamid. . . 



Uric acid 



Hippuric acid 



ALCOHOL 



Ethyl 



SUGAR 



Glucose 



Ethyl 



Diethyl 



Propyl 



Isopropyl.. . 

 Dipropj'l. . . 

 Tripropyl... 



Butyl 



Isobutyl 



Diisobutyl. 



Amyl 



Diamyl 



Ethanol 



Glucosamin 

 Benzyl 



Catalase is formed. 

 Aerobic, facultative. 

 Optimum temperature 30 °C. 

 Habitat : Soil and water. 



Note : The author recognizes four 

 varieties of this species which he dif- 

 ferentiates on the basis of organic sub- 

 stances attacked (see Table) and pig- 

 ment produced. Variety a shows light 

 yellow growth on gelatin, bright red on 

 agar and yellow on amine agar. Variety ^ 

 is light yellow on gelatin, yellow on agar 

 and dark yellow on amine agar. Variety 



7 is light yellow on gelatin, light gray on 

 agar and yellow on amine agar. Variety 



8 is colorless on gelatin and agar and white 

 on amine agar. 



Agar colonies: Circular, red, opaque. 



Amine agar colonies : Circular, dark 

 red. 



The following organic acids are at- 

 tacked : Acetic, lactic, jS-oxybutyric, 

 glycerinic, succinic, malonic, formic, 

 methyl formic, glutaric, maleinic, fu- 

 maric, malic, tartaric, citric and quinic. 



The following amino compounds are 

 attacked: Sarcosin, betain, hippuric 

 acid, asparagine, propionamid, caprona- 

 mid, lactamid, succinamid, allantoin 

 and uric acid. 



Glucose is fermented. 



Catalase is formed. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Habitat : Soil and water. 



