196 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



after long-continued growth on artificial 

 media. 



2. Vibrio berolinensis Neisser. (Arch, 

 f. Hyg., 19, 1S93, 200; Microspira bero- 

 linensis Migula, in Engler and Prantl, 

 Die natiirl. Pflanzenfam., 1, la, 1895, 33.) 

 From M. L., the genitive of Berolina, the 

 Latin name for Berlin. 



Curved rods, somewhat smaller than 

 Vibrio comma. Frequently occurring 

 in pairs. Motile, possessing a polar 

 flagellum. Pleomorphic. Gram-nega- 

 tive. 



Gelatin colonies: Small, grayish, 

 slightly granular, fragmented. 



Gelatin stab: Slow, napiform lique- 

 faction. 



Agar slant: Grayish-yellow, moist, 

 glistening. 



Broth: Turbid, with gray pellicle. 



Litmus milk: No coagulation, no acid. 



Potato: Brownish streak. 



Indole is formed. 



Nitrites produced from nitrates. 



Not pathogenic for mice, pigeons or 

 guinea pigs. 



Aerobic, facultative. 



Optimum temperature 37 °C. Mini- 

 mum above 10 °C. Maximum less than 

 60 °C. 



Source : Isolated from filtered Spree 

 river water. 



3. Vibrio metschnikovii Gamaleia. 



(Gamaleia, Ann. Inst. Pasteur, 2, 1888, 

 482; Pacinia meischmkoffi. Trevisan, I 

 generi e le specie delle Battcriacee, 1889, 

 23; Spirillum metschnikovi Sternberg, 

 Man. of Bact., 1893, 511; Vibrio nord- 

 hafen Pfuhl, Ztschr. f. Hyg., 22, 1894, 

 234; Microspira metschnikoffii Migula, 

 in Engler and Prantl, Die nattirl. Pfian- 

 zenfam., 1, la, 1895, 33.) Named for 

 Metschnikoff, Russian bacteriologist. 



Probable synonyms : Vibrio schuyl- 

 kiliensis Abbott, Jour. Exp. Med., 1, 

 1896, 424 {Microspira schxylkiliensis 

 Chester, Manual Determ. Bact., 1901, 

 334); Vibrio dannbicus Heidcr, Cent. f. 



Bakt., H, 1893, 341 {Microspira danubica 

 Migula in Engler and Prantl, Die natiirl. 

 Pflanzenfam., 1, la, 1895, 33; Spirillum 

 danubicum Holland, Jour. Bact., 5, 1920, 

 225). 



Curved rods, somewhat shorter and 

 thicker than Vibrio comma. Motile. 

 Gram-negative. 



Gelatin colonies : Like those of Vibrio 

 comma. 



Gelatin stab: Rapid, napiform lique- 

 faction. 



Agar slant: Yellowish, plumose, moist, 

 glistening. 



Broth: Turbid, with thin, white pel- 

 licle. 



Litmus milk: Acid, coagulated (eighth 

 day); not peptonized. 



Potato: Delicate, brownish growth. 



Indole is formed. 



Nitrites produced from nitrates. 



Pathogenic for pigeons, fowls, and 

 guinea pigs. 



Aerobic, facultative. 



Optimum temperature 37°C. Maxi- 

 mum less than 45°C. 



Source : Isolated from fowl dead of a 

 choleraic disease. 



Habitat: The intestinal contents of 

 chickens, pigeons and other animals suf- 

 fering from a cholera-like disease. 



4. Vibrio tyrogenus (Fliigge) Hol- 

 land. (Kasespirillen, Deneke, Deutsch. 

 med. Wochnschr., 11, 1885, 33; Spirillum 

 tyrogenum Fliigge, Die Mikroorganismen, 

 2 Aufl.. 1886, 386; Pacinia denekei Trevi- 

 san, I generi e le specie delle Batteriacee, 

 1889, 23; Microspira tyrogena Migula, 

 in Engler and Prantl, Die natiirl. Pflan- 

 zenfam., 1, la, 1895, 33; Holland, Jour. 

 Bact., 5, 1920, 225; Vibrio denekei 

 Ilauduroy et al., Diet. d. Bact. Path., 

 1937, 541.) From Greek tyros, cheese; 

 genes, produced from. 



Curved rods, rather smaller and more 

 slender than Vibrio comma, often very 

 long, closely wound spirals. Motile, 

 possessing a polar flagellum. Gram- 

 negative. 



