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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Litmus milk : Slightly acid ; coagulated ; 

 peptonized. 



Potato : Grayish, slimy layer. 



Indole not formed. 



Nitriites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30 °C. 



Source: Isolated from feces of pa- 

 tients suffering from cholera nostras. 



Habitat : Intestinal contents in cholera 

 nostras and cholera infantum. 



8. Vibrio wolfii (Migula) Bergey et al. 

 {Bacillus choleroides Wolf, Mlinch. med. 

 Wochenschr., 40, 1S93, 693; Microspira 

 wolfii Migula, Syst. d. Bakt., 2, 1900, 

 1001; not Microspira choleroides Migula, 

 loc. cit., 992; Bergey et al., Manual, 1st 

 ed., 1923, 80.) Named for Wolf, who 

 first isolated this organism. 



Curved rods and S-shaped forms. 

 Motile. Gram-negative. 



Gelatin colonies: Small, grayish-white, 

 spreading. 



Gelatin stab: Infundibuliform lique- 

 faction. 



Agar slant: Gray, moist layer. 



Broth: Turbid, witli gray pellicle. 



Litmus milk: Acid; coagulated. 



Potato : Yellowish-white layer. 



Blood serum: Rapid liquefaction. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Source : Isolated from cervical secre- 

 tions in chronic endometritis. 



Gelatin colonies: Small, circular, 

 slightly granular, yellowish, becoming 

 brownish. 



Gelatin: Crateriform liquefaction. 



Agar slant: Grayish-white, moist. 



Broth: Turbid, no pellicle formed. 



Litmus milk: Acid; coagulated. 



Potato : Thin, gray layer, spreading. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Source : Isolated from sputum. 



10. Vibrio liquefaciens (Migula) Ber- 

 gey et al. (Bonhoff, Arch. f. Hyg., 19, 

 1893, 248; Microspira liquefaciens Mi- 

 gula, Syst. d. Bakt., 2, 1900, 990; Bergey 

 et al.. Manual, 1st ed., 1923, 81.) From 

 Latin, liquefacio, to make liquid. 



Comma and S-shaped rods. Motile. 

 Gram-negative. 



Gelatin colonies: Circular, with ir- 

 regular margin, surrounded by a rose- 

 colored zone. 



Gelatin stab : Slow, napiform lique- 

 faction. 



Agar slant: Smooth, grayish, plumose. 



Broth : Turbid, with heavy grayish 

 pellicle. 



Litmus milk: Acid; coagulated. 



Potato : Moist, brownish layer. 



Indole is not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Habitat : Water. 



9. Vibrio sputigenus (Migula) Bergey 

 et al. (Vibrio aus Sputum, Brix, Hyg. 

 Rundschau, 4, 1894, 913; Microspira 

 sputigena Mignla, Syst. d. Bakt., f, 1900, 

 981; Bergey ct al.. Manual, 1st ed.. 1923, 

 80.) From Latin, spuo {sputus), spu- 

 tum; -genes, produced from. 



Slightly curved rods, about the same 

 size and form as Vibrio comma, occurring 

 singly, occasionally three or four in a 

 chain. Motile. Possessing a polar fiagel- 

 lum. Gram-negative. 



11. Vibrio strictus Kutscher. (Ztschr. 

 f. Hyg., 19, 1895, 469.) From Latin 

 stringo (strictus), constricted. 



Markedly curved rods, of about twice 

 the size of Vibrio comma. Motile. 

 Gram-negative. 



Gelatin colonies: Small, circular, yel- 

 lowish, with serrate margin. 



Gelatin stab: Slow, napiform to sac- 

 cate liquefaction. 



Agar slant : Growth plumose, moist. 



Broth: Turbid, with gray pellicle. 



