FAMILY PSEUDOMONADACEAE 



199 



Litmus milk: Not coagulated. 

 Potato: Thin, barely visible layer. 

 Blood serum is slowlj' liquefied. 

 Indole is not formed. 

 Nitrites not produced from nitrates. 

 Pathogenic for guinea pigs. 

 Aerobic, facultative. 

 Optimum temperature 37 °C. 

 Habitat : Water. 



12. Vibrio aquatilis Giinther. (Deut- 

 sche med. Woehenschr., 1S92, 1124; 

 M icrospira aquatilis Migula, System der 

 Bakterien, 2, 1900, 993.) From Latin, 

 aquaticus, living in water. 



Curved rods, like Vibrio comma. 

 Motile, possessing a polar flagellum. 

 Gram-negative. 



Gelatin colonies: Circular, brownish, 

 finely granular, entire. 



Gelatin stab : Crateriform liquefaction. 



Agar slant : Moist, grayish, glistening. 



Broth: Slightly turbid. 



Litmus milk : Not coagulated. 



Potato: No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30 °C. 



Habitat : Water. 



13. Vibrio neocistes Gray and Thorn- 

 ton. (Gray and Thornton, Cent. f. 

 Bakt., II Abt., 73, 1928, 92.) From 

 Greek neos, new and kiste bo.x or ark. 

 Here used as the equivalent of Newark, 

 the name of a city in England. 



Curved rods: 0.5 to 1.0 by 1.0 to 4.0 

 microns. Motile with one to three 

 polar flagella. Gram stain not recorded. 



Gelatin colonies : Liquefied. 



Gelatin stab : Liquefied. Medium red- 

 dened. 



Agar colonies : Circular or amoeboid, 

 buff to brownish, convex, smooth, glis- 

 tening, entire. 



Agar slant :Filiform, fluorescent, raised, 

 smooth, glistening, undulate. 



Broth: Turbid. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Acid from glucose. 

 Attacks naphthalene. 

 Aerobic, facultative. 

 Optimum temperature. 

 Habitat: Soil. 



14. Vibrio cuneatus Gray and Thorn- 

 ton. (Gray and Thornton, Cent. f. Bakt., 

 II Abt., 73, 1928, 92.) From Latin, 

 cuneo (cuneatus) wedge. 



Curved rods : 1.0 by 1.0 to 3.0 microns, 

 the cells tapering at one extremity. 

 Motile with one to five polar flagella. 

 Gram-negative. 



Gelatin colonies: Liquefied. 



Gelatin stab: Liquefied. 



Agar colonies : Circular to amoeboid, 

 white to buff, fiat to convex, smooth, 

 translucent, border entire. 



Agar slant: Filiform, whitish, smooth, 

 glistening. 



Indole not recorded. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



No acid from carbohydrate media. 



Attacks naphthalene. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Source : One strain isolated from 

 soil from Rothamsted, England. 



Habitat: Soil. 



15. Vibrio granii (Lundestad) Stanier. 

 (Bacterium granii Lundestad, Cent. f. 

 Bakt., II Abt., 75, 1928, 330; Achromo- 

 bacter granii Bergey et al., Manual, 3rd 

 ed., 1930, 222; Stanier, Jour. Bact., 42, 

 1941, 538.) Named for Prof. H. H. Gran, 

 who first detected agar-liquefying bac- 

 teria. 



Rods: 0.6 to 0.8 by 1.4 to 2.4 microns, 

 with rounded ends, occurring singly, in 

 pairs, and at times in short chains. 

 Motile. Polar flagellate (Stanier, loc. 

 cit.). Gram-negative. 



Fish-gelatin colonies : Punctiform, 

 black, glistening. 



Fish-gelatin stab : Slow, crateriform 

 liquefaction. 



Sea-weed agar colonies: Circular, flat 



