200 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



opaque, glistening, white, slimy, entire. 

 Agar is dissolved. 



Fish-agar slant: Flat, white, elevated, 

 glistening, undulate. Liquefied. 



Broth: Turbid with grayish-white, 

 slimy sediment. 



Indole not formed. 



Nitrites not produced from nitrates. 



Starch usually hydrolyzed. 



No action on sugars. 



Aerobic, facultative. 



Optimum temperature 20° to 25 °C. 



Source : Sea water of Norwegian Coast. 



Habitat: Presumably sea water and 

 on sea weeds. 



16. Vibrio leonardii Metalnikov and 

 Chorine. (Ann. d. I'Inst. Pasteur, ^2, 

 1928, 1647.) Named for Leonard. 



Curved rods with rounded ends, 0.5 

 to 1.0 by 2.0 to 3.0 microns. Motile 

 with 1 to 3 polar flagella. Gram-negative. 



Gelatin stab : No liquefaction. 



Agar colonies: Small, transparent, cir- 

 cular, having a characteristic odor. 



Broth: Turbid, with thin pellicle. 



Litmus milk: No coagulation, acid, 

 with reduction of litmus. 



Potato: Slight, colorless growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Blood serum not liquefied. 



Hydrogen sulfide formed. 



Acid and gas from glucose, fructose, 

 galactose, lactose, sucrose and mannitol. 

 No acid or gas from maltose or glycerol. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Habitat : Highly pathogenic for insects 

 as Galleria mellonella L. (bee moth), 

 and Pyrausta nubialis Hiibn. (European 

 corn borer). 



17. Vibrio agarliquefaciens (Gray and 

 Chalmers) Bergey et al. (Microspira 

 agar-liquefaciens Gray and Chalmers, 

 Ann. Appl. Biol., 11, 1924, 325; Manual, 

 4th ed., 1934, 119.) From Latin, lique- 

 facio, liquefying; Malay, agar, a jelly 

 from seaweeds. 



Short curved rods, usually c-shaped, 



with occasional s-shaped and coccoid 

 forms. Cells 2.0 microns long by 0.5 

 to 0.7 micron broad; 3.0 to 5.0 microns 

 long in division stages. Coccoid forms 

 stained, 0.5 to 0.7 micron long. Motile 

 with a single polar flagellum. Gram stain 

 not reported. 



Gelatin stab : Very slight surface 

 growth after one month ; the streak then 

 shows a beaded line. No liquefaction. 



Agar colonies : Surface colonies appear 

 as a whitish growth in a depression, sur- 

 rounded by a white ring. The colony is 

 later surrounded by a ring of liquid agar. 

 Deep colonies show a clear area and may 

 be irregular, oval or angular. 



Agar slant : A deep groove is cut along 

 the inoculation streak, whitish growth 

 along sides. The gel is later much weak- 

 ened. 



Broth: Slightly turbid. No pellicle. 



Acid from glucose, lactose and mal- 

 tose. No acid from sucrose or glycerine. 



Utilizes ammonia salts as a source of 

 nitrogen. 



Decomposes cellulose and agar. The 

 presence of one per cent glucose pre- 

 vents the liquefaction of agar. 



Nitrites produced from nitrates. 



Starch is hydrolyzed. 



Aerobic. 



Temperature relations: Optimum 25° 

 C, will grow at 16° but not at 34 °C. 



Habitat: Soil. 



18. Vibrio cyclosites Gray and Thorn- 

 ton. (Gray and Thornton, Cent, fiir 

 Bakt., II Abt., 73, 1928, 92.) From 

 Greek kyklos, circle or ring; sited, to eat; 

 M. L. cyclosites, feeding on rings, i.e. ring 

 compounds. 



Curved rods: 0.5 to 1.0 by 1.5 to 4.0 

 microns. Motile with a single polar 

 flagellum. Gram-negative. 



Gelatin colonies : Circular, buff to 

 brown, flat, smooth, glistening, entire. 



Gelatin stab: No liquefaction. 



Agar colonies : Circular to irregular, 

 pale buff (later greenish), smooth, entire. 



Agar stab: Filiform, greenish buff, 

 raised, smooth, undulate. 



