FAMILY PSEUDOMOXADACEAE 



201 



Broth : Turbid. 



Indole not reported. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Acid from glucose. 



Attacks phenol and ?n-cresol. 



Aerobic, facultative. 



Optimum temperature 30° to 35 °C. 



Habitat : Soil. 



19. Vibrio percolans Mudd and War- 

 ren. (Jour, of Bact., 8, 1923, 447.) 

 From Latin, percolo (percolatus) , filter- 

 ing. 



Curved rods: 0.3 to 0.4 by 1.5 to 1.8 

 microns, occurring singly or in short 

 chains. Pleomorphic. Actively motile 

 by means of 1 to 3 polar flagella. Gram- 

 negative. 



Gelatin stab: Xo liquefaction. 



Agar colonies : Circular, slightly con- 

 vex, amorphous, entire. 



Agar slant : Bluish-white, glistening, 

 streak. 



Broth: Turbid. Pellicle, sediment. 



Litmus milk : L'nchanged. 



Potato: White, slimy streak. 



Indole not formed. 



Nitrites not produced from nitrates. 



Blood serum not licjucfied. 



Starch not hydrolj'zed. 



No action on carbohydrates. 



Passes through bacterial filters. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Non-pathogenic. 



Source: Isolated from haj^ infusion. 



Habitat: Water. 



20. Vibrio andoi Aoi and Orikura. 

 (Cent. f. Bakt., II Abt., 74, 1928, 331.) 

 Named for Andoi, a Japanese scientist. 



Curved rods, with more or less tapering 

 ends, c- or s-shaped, 0.5 to 0.8 by 1.5 to 

 2.5 microns. Motile, with a single polar 

 flagellum. Gram-negative. 



Gelatin: No growth. 



Agar media : No growth. 



Broth: No growth. 



Litmus milk : No growth. 



Potato : No growth. 



Ammonium sulfate agar colonies : 

 Punctiform, circular, concave, surrounded 

 with clear zone. 



Ammonium sulfate agar slant : Grayish, 

 becoming straw-yellow, sinking into the 

 medium as the agar liquefies. 



Cellulose media: No growth. 



Starch hydrolyzed. 



Glucose, fructose, galactose, mannose, 

 xylose and "honyak" are fermented. 



Xylan is decomposed. 



Cellobiose is decomposed. 



Aerobic, facultative. 



Optimum temperature 25 °C. Mini- 

 mum 8 °C . Maximum 37 °C . 



Source : Rotted stable manure. 



Habitat: Presumably decomposing or- 

 ganic matter. 



21. Vibrio fetus Smith and Taylor. 

 (Spirillum causing abortion in sheep, 

 ^lacFadyean and Stockman, Rept. Dept. 

 Comm. Ministr\' Agric. on Epizootic 

 Abortion, London, 1909, 156; ibid., 1913, 

 111 ; Spirillum associated with infectious 

 abortion, Smith, Jour. Exp. Med., 28, 



1918, 701; Smith and Taylor, ihid., 30, 



1919, 299; Spirillum fetus Lehmann and 

 Neumann, Bakt. Diag., 7 Aufl., 2, 1927, 

 552; Vibrio foetus ovis Buxton, First 

 Rept. of Director, L^niv. Cambridge 

 Instit. Animal Pathol., 1929-1930, 47.) 

 From li. foetus, fetus. 



Curved rods : The smallest forms ap- 

 pear as minute curved s-shaped lines, 

 other forms very long; 0.2 to 0.5 by 1.5 

 to 5.0 microns. Motile by means of one, 

 rarely two, polar flagella. Occasionally 

 forms capsules. Granules present in 

 older cultures. Gram-negative. 



Gelatin: Xo growth. 



Agar slant: No surface growth by 

 freshly isolated strains. Laboratory 

 strains produce a scanty, grayish-white, 

 glistening surface growth. 



Subsurface agar colonies : Small, yel- 

 low, opaque. 



Broth : A viscid ring pellicle may ap- 

 pear, faint clouding of the medium occurs ; 

 a filmy, stringy deposit may settle out. 



Litmus milk: No growth 



