202 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato: No growth. 



Indole not formed. 



Nitrite production not reported. 



Blood serum slant: Feeble growth. 

 No liquefaction. 



No gas from carbohydrates. No change 

 or slightly acid from glucose, lactose 

 and sucrose. 



Optimum temperature 37°C. With- 

 stands 55°C for 5 minutes. 



Aerobic or microaerophilic. 



Pathogenesis : Causes abortion in cattle. 



Source : Twenty-two strains isolated 

 from the placentas or fetuses of cows 

 having abortion. 



Habitat: Causes abortion in cattle. 



22. Vibrio pierantonii (Zirpolo) Meiss- 

 ner. {Bacillus pierantonii Zirpolo, Boll. 

 Soc. nat. Napoli, 30, 1918, 206; Meissner, 

 Cent. f. Bakt., II Abt., 67, 1926, 200.) 

 Named for Pierantoni, an Italian bac- 

 teriologist. 



Rods : 0.5 by 1.5 microns, with rounded 

 ends. Motile with one to three polar 

 flagella. Gram-negative. 



Gelatin colonies: Circular, and irregu- 

 larly lobulate. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, light green, 

 smooth, entire. 



Glycerin agar slant : Slightl}^ luminous 

 streak. 



Broth : Turbid, with pellicle. 



Indole not formed. 



Acid from glucose and maltose. 

 Some strains also attack lactose, sucrose 

 and mannitol. 



Best growth in alkaline media. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Source : Isolated from the photogenic 

 organ of the cephalopod Sepiola inter- 

 media Naef . 



Appendix:* The following species have 

 also been listed in the literature. Many 

 are inadequately described. 



Microspira bonhojjli Migula. (Bonhoff , 

 Arch. f. Hyg., 19, 1893, 252; Migula, 

 Syst. d. Bakt., 2, 1900, 1008.) From 

 water. 



Microspira canalis Migula. {Spiril- 

 lum saprophiles y and Vibrio saprophiles 

 7 Weibel, Cent. f. Bakt., 2, 1887, 469; 

 Migula, Syst. d. Bakt., 2, 1900, 1004; 

 Microspira cloaca Chester, Man. Determ. 

 Bact., 1901, 341.) Possibly identical 

 with Microspira saprophiles Migula, 

 Microspira weibelii Migula, Vibrio siirati 

 Ford, Vibrio smithii Ford. From sewage. 



Microspira coprophila Migula. (Group 

 3, No. 6, Kutscher, Ztschr. f. Hyg., 19, 

 1895, 475; Migula, Syst. der Bakt., 2, 

 1900, 986.) From fecal matter. 



Microspira maasei (v. Hoff) Migula. 

 {Spirillum maasei v. Hoff, Cent. f. Bakt., 

 I Abt., 21, 1897, 797; Migula, Syst. d. 

 Bakt., 2, 1900, 978.) Possibly a variety 

 of Vibrio comma Winslow et al. From 

 Rotterdam tap water. 



Microspira milleri Migula. (Miller, 

 Deutsche med. Wchnschr., 11, 1885, 138; 

 Migula, Syst. d. Bakt., 2, 1900, 981; 

 Spirillum milleri Holland, Jour. Bact., 5, 

 1920, 225; Vibrio milleri Holland, ibid.) 

 Probably identical with Vibrio proteus 

 according to Migula. From dental caries. 



Microspira rmirmanensis Issatchenko. 

 (Recherches sur les microbes de I'ocean 

 glacial arctique (in Russian), Petrograd, 

 1914, 240.) From sea water. 



Microspira saprophiles Migula. (Heu- 

 vibrio 13, Weibel, Cent. f. Bakt., 2, 1887, 

 469 ; Vibrio saprophiles /3 Weibel, Cent, f . 

 Bakt., 4, 1888, 225; Migula, Syst. d. 

 Bakt., 2, 1900, 1006; Microspira weibell 

 Chester, Man. Determ. Bact., 1901, 230.) 

 Probably identical with Microspira cloaca 

 Chester and Vibrio surati Ford. From 

 sewage. 



Microspira tyrosinalica Beijerinck. 

 (Kon. Akad. Wetenschappen, Amster- 

 dam, 13, 1911, 1068.) From sewage. 



Microspira weibelii Migula. {Vibrio 



* Prepared by Mr. Wm. C. Ilaynes, New York State Experiment Station, Geneva, 

 New York, Jan., 1939; Revised by Capt. Wm. C. Haynes, Sn. C, Fort Bliss, Texas, 

 July, 1943. 



