224 MANUAL OF DETERMINATIVE BACTERIOLOGY 



ccc. Causes formation of nodules on species of genus Trifolium. Bac- 

 teroids pear-sliaped, swollen, vacuolated. Pentoses usually not 

 fermented. 



3. Rhizobium trijolii. 

 aa. No serum zone formed in milk. 



b. Scant growth, alkaline reaction on yeast water agar plus most carbohydrates. 

 c. Causes formation of nodules on species of genus Lupinus and on Orni- 

 thopus sativus. Bacteroids vacuolated, rods seldom branched. 



4. Rhizohium lupini. 



cc. Causes formation of nodules on Soja max. Bacteroids long slender 

 rods, seldom vacuolated or branched; young cells monotrichous. 



5. Rhizobium japonicum.* 

 2. Litmus milk acid. 



a. Formation of serum zone in milk. 



b. Moderate growth, slight acid reaction on yeast w^ater agar plus mono-, di- 

 and trisaccharides. 



c. Causes formation of root nodules on species of the genera Melilotus, 

 Medicago, and Trigonella. Bacteroids club-shaped, branched, young 



cells peritrichous. 



6. Rhizobium meliloti. 



1. Rhizobium leguminosarum Frank Naturreiche. 2 Theil, Botanik, III Abt., 



emend. Baldwin and Fred. (Frank, Kryptogamen, Sec. 914, 1877, 1944; 



Landwirtschaftliche Jahrbiicher, 79, 1890, Schinzia leguminosarum Frank (all spe- 



563; Rhizobium polymorphum Dangeard, cies), Bot. Ztg., 37, 1879, 377; Phyto- 



Rhizobiumfabae'Dangeard,'LeBotsimste, viyxa leguminosarum Schroeter (all ex- 



Ser. 16, 1926, 192-194 ; Baldwin and Fred, cept Rhizobium lupini), in Cohn, Krypto- 



Jour. Bact., 17, 1929, 146.) From Latin, gamen-Flora von Schlesien, 3, I, 1886, 



of the legume family (Leguminosae) . 135; Bacillus radicicola Beijerinck (all 



species), Bot. Ztg., 46, 1888, 726; Bacillus 



Note: The following binomials have fabae Beijerinck (from broad bean) and 



been used for species of this genus. The Bacillus oi-nilhopi Be'iierinck (from ser- 



names given were used by their authors radella), Bot. Ztg., 48, 1890, 837; Clado- 



to cover one or more of the species here chytriuin tuberculorum Vuillemin (all 



recognized as belonging to the genus species?), Ann. Sci. Agron. Franc, et 



Rhizobium. Where a question mark (?) Etrang., 5, I, 1888, 193; Bacterium 



is used it indicates that the species was radicicola Prazmowski (all species), 



too poorly described to be recognizable Landw. Vers. Sta., 37, 1890, 204; Rhizo- 



today. Schinzia cellulicola Frank, 1877 femmnmiobiZe Schneider (several species) 



(all species) Leunis, Synopsis der drei Rhizobium curvum Schneider (?), Rhizo- 



* No specific name has been proposed for the organism causing the formation of 

 nodules on plants that are members of the so-called "cowpea" group. Data showing 

 possible inter-relationships of certain plant species of the soybean and cowpea cross- 

 inoculation groups prompted Walker and Brown (Soil Science, 39, 1935, 221-225) to 

 propose a consolidation of the two groups to be recognized as being inoculated by a 

 single species, Rhizobium japonicum. Results obtained recently by Reid and Bald- 

 win (Proc. Soil Sci. Soc. Amer. for 1936, 1, 1937, 219) show these inter-relationships to 

 include the lupine group also. 



