FAMILY RHIZOBIACEAE 



233 



Rods: 0.5 to 0.8 by 1.0 to l.-l microns, 

 occurring singlj'. ^lotile with a single 

 or occasionally with peritrichous flagella. 

 Gram-negative. 



Gelatin colonies: Thin, bluish, becom- 

 ing violet, crumpled. 



Gelatin stab: Heavy, violet-black pel- 

 licle. Liquefied. 



Agar colonies: Deep violet, surface 

 rugose. 



Agar slant: Thick, moist, yellowish- 

 white, becoming violet with metallic 

 luster. 



Broth: Pellicle with violet sediment, 

 fluid becoming violet. 



Litmus milk: Violet pellicle. Diges- 

 tion turning alkaline. 



Potato: Deep violet, rugose spreading 

 growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Usually no acid from glucose, maltose 

 and sucrose. No acid from lactose. 



Aerobic, facultative. 



Optimum temperature 30°C. No 

 growth at 37°C. Good growth in 7 days 

 at 2 to 4°C. 



Original source : Found once by Jolles 

 in spring water from Spalato. 



Habitat: Water. 



Appendix: The following organisms 

 have been assigned to this genus or are 

 believed to belong here. Additional 

 comparative studies are badly needed. 



Bacillus cyaneo-fuscus Beijerinck. 

 (Beijerinck, Bot. Ztung., .',9, 1891, 704; 

 Bacterium ci/anojuscus Chester, Ann. 

 Kept. Del. Col. Agr. Exp. Sta., 9, 1897, 

 116 and 132.) From black glue, blue 

 Edam cheese, water and soil. 



Bacillus lacyyius Schroeter. (Schroeter 

 in Cohn, Kryptogamen-Flora von Schle- 

 sien, 3, 1, 1889, 158.) In greenhouse on 

 fresh paint. 



Bacillus lilacinus Mace. (Traite Pra- 

 tique Bact., 6« ed., 2, 1913, 416.) From 

 water. 



Bacillus tuembranuceus umethystinus 

 inobilis Germano. (Gcrmano, Cent. f. 

 Bakt., 12, 1892, 516; Bacillus amethysti- 



nus mobilis Kruse, in Fliiggc, Die 

 Mikroorganismen, 3 Aufl., 2, 1896, 313; 

 Bacterium amethystinus mobilis Chester, 

 Ann. Kept. Del. Col. Agr. Exp. Sta.^ 

 9, 1897. 117; Bacteria?/! memhranaceus 

 mobilis Chester, ibid.. 138.) Pseudo- 

 monas amethyslina Migula, Syst. d. 

 Bakt., 2, 1900, 944; Bacillus amethys- 

 tinus Chester, Man. Determ. Bact.. 1901, 

 262.) From dust. 



Bacillus pavoninus Forster. (Forster, 

 in van der Sleen, Sur I'examen bacterio- 

 logique qualitatif de I'eau. Arch. Teyler, 

 Ser. 2, Tome 4, 3 partie, 1894, No. 59, 

 Haarlem, Heritiere Loosjes. Also see 

 Godfrin, These, Nancy, 1934, 46.) 

 Causes blue discoloration of Edam cheese. 



Bacillus polychromogoies Chamot and 

 Thiry. (Bacille polychrome, Thiry, 

 Compt. rend. Soe. Biol., Paris, 48, 1896, 

 885; Chamot and Thiry, Bot. Gaz., 30, 

 1900, 378.) From well water at Nancy. 

 Probably a Pseudomonas (Tobie, per- 

 sonal communication). 



Bacillus violaceus Frankland and 

 Frankland. (Frankland and Frankland, 

 Ztschr. f . Hyg., e, 1888, 394 ; Pseudomonas 

 pseudoianthina IMiguIa, Syst. d. Bakt., 

 2, 1900, 942.) Isolated from tap water. 

 Said to produce spores. 



Bacillus violaceus laurentius Jordan. 

 (Jordan, Mass. State Bd. Health Rept., 

 1890. 838; Bacterium violaceus laurentius 

 Chester, Ann. Rept. Del. Col. Agr. 

 Exp. Sta., .9, 1897, 117; Pseudomonas 

 laurentia Migula, Syst. d. Bakt., 2, 

 1900, 944; Bacillus violaceus Chester, 

 Man. Determ. Bact., 1901, 262; Chromo- 

 bacterium violaceum laurentium Ford, 

 Textb. Bact., 1927, 470.) Isolated from 

 sewage effluent. 



Bacillus violaceus lutetiensis Kruse. 

 (Kruse, in Fliigge, Die Mikroorganismen, 

 3 Aufl., 2, 1896, 311; Bacillus lutetiensis 

 Chester, Man. Determ. Bact., 1901, 306; 

 Chromobacterium violaceum lutetiense 

 Ford, Textb. Bact., 1927, 470.) From 

 water. 



Bacillus violaceus sarloryi Waeldele. 

 (These, Pharm. Strasbourg, 1938, 55.) 



