240 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Agar colonies : Luxuriant, moist, sulfur 

 yellow. 



Agar slant: Light yellow, plumose, 

 slightly rugose, somewhat dull, raised 

 center and transparent margin. 



Broth: Turbid, with light orange ring 

 and sediment. 



Milk: Generally acid but not suffi- 

 cient to curdle. 



Potato: No growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Blood not hemolyzed. 



Starch not hydrolyzed. 



Acid from glucose and lactose gen- 

 erally, sometimes from sucrose. Manni- 

 tol and glycerol generally not fermented. 



Ammonia produced from peptone. 



Utilizes NH4H2PO4 as a source of 

 nitrogen. 



Resistant to drying and heat. 



Non-pathogenic. 



Aerobic . 



Optimum temperature 25°C. 



Source : Found in gonorrhoeal pus and 

 dust. 



Habitat: Infections, milk, dairy prod- 

 ucts, dairy utensils, water, common. 



7. Micrococcus varians Migula. 

 (Merismopedia Jlava varians Dyar, Ann. 

 N. Y. Acad. Sci., 8, 1895, 346; Migula, 

 Syst. d. Bakt., 2, 1900, Ido; Merismopedia 

 flava-varians Chester, Man. Determ. 

 Bact., 1901, 103; Micrococcus lactis vari- 

 ans Conn, Esten and Stocking, Storrs 

 Agr. Exp. Sta. Rept. for 1906, 121.) From 

 Latin, varians varying. 



Spheres: 0.8 to 1.0 micron, occurring 

 singly, in pairs and in fours. Occa- 

 sionally cultures are found that are motile 

 with a single flagellum. Otherwise non- 

 motile. Gram -variable. 



Gelatin colonies: Small, circular, whit- 

 ish to yellow, capitate, moruloid. 



Gelatin stab : Scant growth. No lique- 

 faction. 



Agar colonies: Small, yellow, raised, 

 glistening. 



Agar slant: Plumose, yellow, vari- 

 egated. 



Broth: Turbid, with yellow, granular 

 sediment. 



Litmus milk : Acid; coagulated on boil- 

 ing. 



Potato: Raised, dry, bright-yellow, 

 glistening. 



Indole not formed. 



Nitrites produced from nitrates. 



Acid from glucose, lactose, sucrose, 

 raffinose and frequently from glycerol 

 and mannitol. No acid from salicin or 

 inulin. 



Starch not hydrolyzed. 



Ammonia produced from peptone. 



Utilizes NH4H2PO4 as a source of 

 nitrogen. 



Saprophytic. 



Aerobic. 



Optimum temperature 25°C. 



Source : Original strains found in a 

 contaminated jar of sterilized milk. 



Habitat : Has been found in body 

 secretions, dairy products, dairy utensils, 

 dust and water, including sea water. 



8. Micrococcus caseolyticus Evans. 

 (Evans, Jour. Inf. Dis., 18, 1916, 455; 

 Micrococcus casei Hucker, N. Y. Agr. 

 Exp. Sta. Tech. Bull. 102, 1924, 17; Prob- 

 ably Micrococcus casei Holland, Jour. 

 Bact., 5, 1920, 223.) 



Identical in part with Micrococcus casei 

 acidoproteolyticus I and // Gorini, Rev. 

 Gen. du Lait, 8, 1910, 337; Tetracoccus 

 liquejaciens Orla-Jensen, The Lactic 

 Acid Bacteria, 1919, 80 {Micrococcus casei 

 liquefaciens Orla-Jensen, Doktordispu- 

 tats, 1904; Tetracoccus casei liquefaciens 

 Orla-Jensen, The Lactic Acid Bacteria, 

 1919, 80 ; Micrococcus liquefaciens Holland , 

 Jour. Bact., 5, 1920, 224. Also see ref- 

 erences under Streptococcus liquefaciens.) 

 From Latin, caseus, cheese, casein; and 

 Greek, lyticus, able to dissolve; M. L., 

 dissolving, digesting. 



Spheres, variable in size, occurring in 

 clumps. Non-motile. Gram-positive. 



Gelatin stab: Liquefaction generally 



