FAMILY MICROCOCCACEAE 



245 



in irregular masses, generally not singly 

 or in pairs. Non-motile. Gram-nega- 

 tive to Gram-variable 



Gelatin colonies : After several days, 

 small, pink or flesh-colored, shiny, buty- 

 rous, 0.5 to several mm. in diameter. 

 Smaller colonies have regular edges ; 

 larger colonies have lobate edges. 



Gelatin streak: Thick, shiny, flesh- 

 colored to carmine-red growth, generally 

 spreading. 



Gelatin stab: Scant, whitish growth 

 along line of stab; surface growth flesh- 

 red. No liquefaction after several weeks, 

 but a slight softening of the medium 

 underneath the growth. 



Agar slant: Luxuriant, thick, spread- 

 ing, slimy, flesh-colored growth. 



Broth: Bright red, slimy sediment. 

 No pellicle. 



Milk: General!}' acid curd followed by 

 slight peptonization. 



Nitrites produced from nitrates. 



Acid from glucose, sucrose, mannitol 

 and glycerol. No action on lactose or 

 starch. 



Pigment soluble in ether, benzol, car- 

 bon bisulfide, chloroform and alcohol. 

 Not soluble in water (Schneider, loc. 

 cit.). 



Saprophytic. 



Grows well at 26" to .37°C. 



Aerobic. 



Source : Original culture isolated by 

 Bujwid in Bern, Switzerland and sent to 

 Migula at Karlsruhe, Germany. 



Habitat: Unknown. 



16. Micrococcus rhodochrous (Zopf) 

 -Migula. (Rhodococcus rhodochrous Zopf, 

 Berichte d. deutsch. bot. Gesellsch., 9, 

 1891, 22; Migula, Syst. d. Bakt., 3, 1900, 

 162.) From Greek, rhodiim, rose; chros, 

 color. 



Spheres: 0.5 to 1.0 micron, occurring 

 singly. Non-motile. Gram-variable. 



Gelatin colonies: Small, circular, glis- 

 tening, raised, entire, dark, reddish- 

 brown . 



Gelatin stab: Dark, carmine-red, dry 

 surface growth. Slight growth in stab. 

 No liquefaction. 



Agar slant : Carmine-red streak, be- 

 coming brick-red in color. 



Broth : Thick rose-red pellicle with 

 red, flocculent sediment. 



Litmus milk : Slightly alkaline. 



Potato: Carmine-red streak. 



Does not ferment glycerol and man- 

 nitol. 



Aerobic. 



Saprophytic. 



Optimum temperature 25°C. 



Habitat : Water. 



17. Micrococcus agilis Ali-Cohen. 

 (Ali-Cohen, Cent. f. Bakt., 6, 1889, 36; 

 Planosarcina agilis Migula, in Engler 

 and Prantl, Die naturl. Pflanzenfam., 1, 

 la, 1895, 20; Micrococcus agilis ruber 

 Peppier, Cent. f. Bakt., I Abt., 29, 1901, 

 352; Planococcus agilis Chester, Man. 

 Determ. Bact., 1901, 115; Rhodococcus 

 agilis Winslow and Rogers, .Jour. Inf. 

 Dis., 3, 1906, 545; Sarcina agilis Ender- 

 lein, Sitzber. Gesell. Naturf. Freunde 

 Berlin, 1930, 182; not Sarcina agilis 

 Matzuschita, Zeit. f. Hyg., 35, 1900, 

 496; not Sarcina agilis Saito, Jour. Coll. 

 Sci. Imp. Univ. Tokyo, 23, 1908, .) 

 From Latin, agilis, agile. 



Spheres, 1.0 micron, occurring singly, 

 in pairs and in fours. Motile by means 

 of one or two flagella. Gram-variable. 



Gelatin colonies : Small, gray, becoming 

 distinctly rose-colored. 



Gelatin stab: Thin, whitish growth in 

 stab. On surface thick, rose-red, glis- 

 tening growth. Generally no liquefac- 

 tion . 



Agar slant: Glistening, dark rose-red, 

 lobed, much variation in color. 



Broth: Slightly turbid, with slight, 

 rose-colored ring and pink sediment. 



Litmus milk: Slightly acid, pink sedi- 

 ment . 



Potato: Slow growth as small, rose- 

 colored colonies. 



Loeflier's blood serum: Pink, spread- 



