246 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



ing, shiny, abuudaut. Slow liquefaction. 



Indole not formed. 



Nitrites produced (trace). 



Ammonia formed (trace). 



Does not utilize NH4H2PO4 as source 

 of nitrogen. 



Acid from glucose, sucrose, inulin, 

 glycerol and mannitol. No acid from 

 raffinose . 



Aerobic. 



Saprophytic. 



Optimum temperature 25°C. 



Source : Isolated from water. 



Habitat : Water, sea water, on sea fish. 



*18. Micrococcus aerogenes (Schott- 

 miiller) Bergey et al. {StapMjlococcus 

 aerogenes Schottmliller, Cent. f. Bakt., I 

 Abt., Orig., 64, 1912, 270; Bergey et al., 

 Manual, 1st ed., 1923, 70; not Micrococcus 

 aerogenes Miller, Deutsch. mod. 

 Wchnschr., 12, 1886, 119.) From Greek, 

 forming air or gas. 



Description according to Prevot, Ann. 

 Sci. Nat., Ser. Bot. et Zool., 15, 1933, 

 212. 



Spheres: 0.6 to 0.8 micron, occurring 

 in clusters, sometimes in pairs or short 

 chains. Gram-positive. 



Gelatin: No liquefaction. 



Deep agar colonies: Small, lenticular, 

 nearly spherical, yellowish white. Some 

 gas bubbles produced, not fetid. 



Blood agar colonies: Very small, gray- 

 ish. No true hemolysis, but a narrow 

 clear zone is formed. 



Serum agar: Colonies lenticular. Gas 

 not fetid. 



Neutral red serum agar : Colonies len- 

 ticular. Gas produced. Neutral red 

 changed to greenish yellow. 



Glucose broth with blood: Turbid. 

 Gas produced. Hydrogen sulfide not 

 produced. Slight hemolysis. 



Glucose serum broth: Turbid. Gas 

 produced. 



Peptone water with serum : Gas . Indole 

 produced. 



Milk: Growth feeble. Neither acid 

 nor coagulated. 



Proteins not attacked. 



Glucose and fructose attacked slightly 

 by two out of three strains. 



Does not plasmolyse readily. 



Neutral red broth : Changed to yellow- 

 ish green. 



Nitrites not produced from nitrates. 



Optimum pH 6.5 to 8.0. 



Optimum temperature 37°C. 



Pathogenic. 



Strict anaerobe. 



Distinctive character: Fermentation 

 of glucose and gas production from 

 peptones. 



Source : Isolated (Schottmliller) from 

 cases of puerperal fever. Three strains 

 from infected tonsils studied by Prevot. 



Habitat : Natural cavities, especially 

 the tonsils and female genital organs. 



19. Micrococcus asaccharolyticus (Dis- 

 taso) comb. nov. {Staphylococcus asac- 

 charolyticus Distaso, Cent. f. Bakt., I 

 Abt., Orig., 62, 1912, 445.) From Greek, 

 not dissolving sugar. 



Description according to Prevot, Ann. 

 Sci. Nat., Ser. Bot., 15, 1933, 211. 



Large spheres: 1.0 to 1.2 microns, 

 occurring in very large clusters, also in 

 pairs and short chains. Gram-positive. 



Gelatin: At 37°C, growth resembles 

 tufts of cotton wdiich precipitate. No 

 liquefaction. 



Deep agar colonies: Very delicate, 

 pin-point, transparent. A few bubbles 

 of gas produced. 



Broth: Turbid. Growth settles at 

 the bottom of the tube as a sort of vis- 

 cous zooglea. Unpleasant odor pro- 

 duced. 



Peptone water: Turbid. Indole pro- 

 duced. 



Milk: Feebly acidified, but not 

 coagulated. 



Egg white not attacked. 



Carbohydrates not attacked. 



Strict anaerobe. 



Anaerobic section revised by Dr. Ivan C. Hall, New York, N. Y. 



