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MANUAL OF DETERMINATIVE BACTERIOLOGY 



3. Neisseria catarrhalis (Frosch and 

 Kolle) Holland. {Micrococcus catarrh- 

 alis Frosch and Kolle, in Fliigge, Die 

 Mikroorganismen, 3 Aufl., 2, 1896, 154; 

 Diplococcus pharyngis communis von 

 Lingelsheim, Klin. Jahrb., 15, 1906, 408; 

 Holland, Jour. Bact., 5, 1920, 224.) 

 From Greek, catarrh, a running down. 



Spheres : 0.6 to 0.8 micron in diameter, 

 occurring singly or in pairs with adjacent 

 sides flattened, occasionally in fours. 

 Gram-negative. 



Agar colonies: Small, circular, grayish 

 white to dirty white, with erose margins. 



Broth: Turbid, often with slight pel- 

 licle. 



No acid from any of the carbohydrates. 



Optimum tempei'ature 37°C. Grows 

 well at 22°C. 



Aerobic, facultative. 



Source: Nasopharynx, saliva and res- 

 piratory tract. 



Habitat : Human mucous membrane of 

 the respiratory tract. Often associated 

 with other organisms in inflammations of 

 the mucous membrane. 



Note: Topley and Wilson (Prin. of 

 Bact., 1931, 349) state that Neisseria 

 pharyngis cinerea (Micrococcus pharyngis 

 cinereus von Lingelsheim, Klin. Jahrb., 

 15, 1906, 373) resembles Neisseria catar- 

 rhalis so closely that it should probably be 

 regarded as a variety of this species. 



4. Neisseria sicca (von Lingelsheim) 

 Bergey et al. {Diplococcus pharyngis 

 siccus von Lingelsheim, Klin. Jahrb., 15, 

 1906, 409; Diplococcus siccus von Lingels- 

 heim, Ztschr. f. Hyg., 59, 1908, 476; 

 Micrococcus pharyngis siccus Kutscher, 

 in Kolle and Wassermann, Handb. d. 

 Path. Mikroorganismen, 2 Aufl., 4, 1912. 

 603; Micrococcus pharrjngis-siccus Hol- 

 land, Jour, of Bact., 5, 1920, 224; Neis- 

 seria pharyngis-sicci (sic) Holland, ibid.; 

 Bergey et al., Manual, l.st ed., 1923, 43.) 

 From Latin, sicca, dry. 



Spheres : 0.6 to 0.8 micron in diameter, 

 occurring singly and in pairs with ad- 

 jacent sides flattened. Gram-negative. 



Blood agar colonies : Grayish, somewhat 

 dry, crumbling when an effort is made to 

 remove them. 



Ascitic agar colonies: Small, very firm 

 and adherent to medium, becoming cor- 

 rugated on the surface. 



The organisms precipitate spontaneous- 

 ly when suspended in normal salt solu- 

 tion. 



Acid from glucose, fructose, maltose 

 and sucrose. No acid from mannitol. 



Optimum temperature 37°C. Grows 

 at 22°C. 



Aerobic, facultative. 



Source: Nasopharynx, saliva and spu- 

 tum. 



Habitat : Mucous membrane of the 

 respiratory tract of man. 



5. Neisseria perflava Bergey et al. 

 (Chromogenic group I, Elser and Hun- 

 toon, Jour. Med. Res., .20 (N. S. 15), 1909, 

 415; Bergey et al.. Manual, 1st ed., 1923, 

 43.) From Latin per, very and flavus, 

 yellow. 



Spheres: 0.6 to 0.8 micron, occurring 

 singly and in pairs with adjacent sides 

 flattened. Gram-negative. 



Glucose agar colonies: Small, circular, 

 slightly raised, greenish-gray by reflected 

 light, and greenish-yellow and semi- 

 opaque by transmitted light. The sur- 

 face is smooth, glistening. The growth 

 is adherent to the medium. Chromo- 

 genesis best seen on Loffler's blood serum 

 medium. 



Ascitic agar colonies : Like those on 

 glucose agar. 



Acid from glucose, maltose, fructose, 

 sucrose and mannitol. 



Optimum temperature 37°C. Grows 

 at22°C. 



Aerobic, facultative. 



Source : Nasopharynx, saliva and spu- 

 tum. 



Habitat : Mucous membrane of respira- 

 tory tract of man. 



6. Neisseria flava Bergey etal. {Dip- 

 lococcus pharyngis flaims I and possibly 



