300 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Gelatin: No liquefactiou. 



Deep agar colonies : Lenticular, up to 

 1 mm in diameter. Grows in a narrow 

 disk about 1 cm below the surface. Gas 

 produced. 



Broth : Turbid. Fine granular precipi- 

 tate. Slight rancid odor and inflam- 

 mable, explosive gas produced. 



Peptone water: Gas produced. 



Indole not formed. 



No action on milk. 



Coagulated proteins not digested. 



Carbohydrates not attacked. 



Hydrogen sulfide not produced. 



Neutral red glucose broth: Becomes 

 pink, but no further change. 



Optimum pH 7.0 to 8.0. 



Temperature relations : Optimum 37°C. 

 No growth at 28°C. Killed in half an 

 hour at 60°C. 



Non-pathogenic. 



Strict anaerobe. 



Distinctive characters: Colonies grow 

 in narrow zone 1 cm below the surface 

 of an agar stab; gas produced from pep- 

 tones. 



Source : Isolated from bronchial mucus, 

 respiratory system ; dental and tonsillary 

 focal infections. 



Habitat: Buccal cavity (human) and 

 probably also in other warm-blooded 

 animals. 



10. Neisseria reniformis (Cottet) Tre- 

 vot. {Diplococcus reniformis Cottet, 

 Compt. rend. Soc. Biol., 52, 1900, 421; 

 Micrococcus reniformis Oliver and Wher- 

 ry, Jour. Inf. Dis., 28, 1921, 341 ; Prevot, 

 Ann. Sci. Nat., Ser. Bot., 15, 1933, 102.) 

 From Latin, ren (renes) , kidney ; -formis , 

 form, i.e. kidney-shaped. 



Spheres :0.8 to 1 .0 micron, bean-shaped, 

 occurring in pairs. Gram-negative. 



Gelatin : No liquefaction. 



Deep agar colonies : Appear in 24 to 48 

 hours; at first punctiform, then lenti- 

 cular; small, 0.3 to 0.5 mm. No gas 

 produced. 



Agar slant : Minute, bluish-white, dew- 

 drop colonies. 



Broth : Turbid in 24 hours ; flocculent 

 precipitate rapidly formed, clearing the 

 medium. No gas produced, but a rancid 

 odor is present. 



Peptone water: Very meagre growth. 

 Traces of indole. 



Milk: Unchanged. 



Coagulated proteins not digested. 



Slight amount of acid from glucose by 

 one strain only. 



Optimum pH 7.0. Limits of pH 6.0 

 to 8.0. 



Temperature relations : Optimum 37°C. 

 No growth at 22°C. Killed in half an 

 hour at 60°C. or in an hour at 56°C. 



Pathogenic. 



Strict anaerobe. 



Distinctive character : Odor of rancid 

 butter. 



Source : Isolated in several cases from 

 suppurations of the urino-genital system. 



Habitat : Presumably in bodies of 

 warm-blooded animals. 



11. Neisseria orbiculata Prevot. (Dip- 

 lococcus orbicuhts Tissier, Ann. Inst. 

 Past., 22, 1908, 204; Prevot, Ann. Sci. 

 Nat., Ser. Bot., 15, 1933, 109.) From 

 Latin, orbiculatus, having the form of 

 an orb or sphere. 



Spheres: 1.5 to 2.0 microns, occurring 

 in pairs. Gram-negative. 



Gelatin: No growth at 22°C. 



Deep agar colonies : After 36 to 48 hours , 

 large, lenticular, very regular, whitish, 

 almost transparent. Gas not produced. 



Broth : Turbid. Sediment. 



Milk : No coagulation. 



Egg white not attacked. 



Proteoses attacked without formation 

 of indole. 



Acid from glucose. Acid produced 

 feebly from lactose. No acid from su- 

 crose. 



Temperature relations : Optimum 37°C. 

 No growth at 22°C. Killed at 60°C. 



Non-pathogenic. 



Strict anaerobe. 



Distinctive characters : Large size ; no 

 gas production . 



