310 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Diplococcus pneumoniae but is a strict 

 anaerobe ; highly pathogenic . 



Source: Isolated by Rist (loc. cit.) 

 from an osseous abscess; by Bolognesi 

 (Joe. cit.) from lesions of pleuropneu- 

 monia. 



Habitat : Buccal-pharyngeal cavity of 

 man and rodents. 



4. Diplococcus plagartim-belli Prevot. 

 (Diplococcus from septic wounds, Adam- 

 son, Jour. Path, and Bact., 22, 1919, 393; 

 Prevot, Ann. Sci. Nat., Ser. Bot., 15, 

 1933, 157.) From Latin plaga, wound; 

 belluni, war. 



Spheres: 0.6 to 1.0 micron, occurring 

 in pairs of unequal size or in short chains. 

 Gram-positive. 



Gelatin: No liquefaction. 



Deep agar colonies : Appear after 24 to 

 48 hours, gradually increasing in size to 

 2 mm in diameter; lenticular, regular, 

 almost transparent. Gas not produced, 

 even in glucose agar. 



Broth: Growth precipitates in 5 or 6 

 days. No gas produced. 



Indole not formed. 



Milk : Strongly acidified and coagu- 

 lated in 2 to 3 days. 



Serum not digested. 



Acid but not gas from glucose, mal- 

 tose, lactose and sucrose. Xo acid from 

 mannitol. 



Temperature relations : Optimum 

 37°C. Not always killed in half an hour 

 at 80°C. 



Non-pathogenic . 



Strict anaerobe. 



Source : Si.xteen strains isolated from 

 fifty -one cases of septic war wounds. 



Habitat: Common in septic wounds. 



5. Diplococcus constellatus Prevot. 

 (Compt. rend. Soc. Biol. Paris, 91, 1924, 

 426.) From Latin constellatus, studded 

 with stars. 



Description in part from Prevot, Ann. 

 Sci. Nat., Ser. Bot., 15, 1933, 158. 



Spheres : 0.5 to 0.6 micron, occurring in 

 pairs and tetrads, rarely in very short 



chains, never in clusters. Gram- 

 positive. 



Gelatin: Good growth. No lique- 

 faction. 



Deep agar colonies : At first very small, 

 lenticular, biconvex, thick, opaque, 

 yellowish, 0.5 to 1.5 mm in diameter. 

 Each colony surrounded by many small 

 satellite colonies visible microscopically. 



Broth: Growth slow, poor. After 48 

 hours a slight homogenous turbidity 

 which quickly clears, leaving a slight 

 powdery sediment. Neither gas nor odor 

 produced. 



Glucose broth : Growth rapid, 

 abundant. 



Proteins not attacked. 



Blood broth: Good growth. No 

 hemolysis. 



Milk: Poor growth. No change. 



Peptone water: Good growth. Not 

 acidified. Indole not formed. 



Neutral red broth unchanged. 



Acid but not gas from glucose, 

 arabinose. Slightly acid from glycerol. 

 No acid from lactose, inulin, mannitol or 

 dulcitol. 



Optimum pH 6.0 to 8.0. 



Optimum temperature 37°C. Feeble 

 growth at 22°C. Not thermo-resistant. 



Strict anaerobe. 



Distinctive character : The micro- 

 scopic appearance of agar colonies each of 

 which is surrounded by a constellation of 

 satellites. 



Source : Isolated from a case of chronic, 

 cryptic tonsillitis. Later isolated from 

 pus in acute appendicitis. 



Habitat: Digestive tract, especially 

 the lymphoid tissues, as tonsils and 

 appendix. 



6. Diplococcus morbillorum Prevot. 



(Diplococci from cases of measles, 

 Tunnicliff, Jour. Amer. Med. Assoc, 68, 

 1917, 1028; Diplococcus rubeolae Tunni- 

 cliff, Jour. Inf. Dis., 52, 1933, 39; Prevot, 

 Ann. Sci. Nat., Ser. Bot., 15, 1933, 148; 

 original name withdrawn by Tunnicliff, 



