314 MANUAL OF DETERMINATIVE BACTERIOLOGY 



hemolytic (though they may be under anaerobic conditions) but show 

 varying degrees of greening of blood. Do not produce ammonia from 

 peptone (few exceptions in Streptococcus mitis). 



1. Lactose is fermented. 



a. Do not grow at 50°C. Greening or indifferent in blood agar. Raf- 



finose, inulin, salicin and dextrin generally fermented. Esculin 



generally attacked. Growth with 2 per cent NaCl. 



b. Do not survive 60°C for 30 minutes. Starch not hydrolyzed. 



Not tolerant of bile. 



c. Mucoid colonies produced on sucrose and raffinose media. 



6. Streptococcus salivarius. 



cc. Colonies not mucoid on sucrose or raffinose media. Inulin 

 not fermented. 



7. Streptococcus mitis. 



bb. Survives 60°C for 30 minutes. Starch is hydrolyzed except 

 by variety inulinaceus. Tolerant of bile. 



8. Streptococcus bovis (and varieties). 



aa. Grows at 50°C. No action on blood. Esculin not attacked. Raf- 

 finose, inulin, salicin and dextrin not fermented. No growth in 

 2 per cent NaCl. 



9. Streptococcus thermophilus . 



2. Lactose not fermented. Tolerant of bile. 



10. Streptococcus equinus. 



C Lactic group. Growth at 10°C. No growth at 45°C. Reduce litmus prior 

 to curdling of litmus milk. Sorbitol and glycerol not fermented. Not 

 beta hemolytic. Tolerate 0.1 per cent methylene blue, but do not 

 tolerate 6.5 per cent NaCl or pH 9.6. 



1. Maltose and dextrin fermented. Ammonia produced from peptone. 



Growth at 40°C. Group N of Shattock and Mattick. 



11. Streptococcus lactis. 



2. Maltose and usually dextrin not fermented. Ammonia not produced 



from peptone. No growth at 40°C. 



12. Streptococcus cremoris. 



D. Enterococcus group. Growth at 10°C. Growth at 45°C. Usually reduce 

 litmus prior to curdling litmus milk. Sorbitol, glycerol and mannitol 

 generally fermented. May or may not be beta hemolytic. Tolerate 

 0.1 per cent methylene blue, 6.5 per cent NaCl and pH 9.6. Ammonia 

 produced from peptone. Lancefield Group D. 



1. Not beta hemolytic. 



a. Gelatin not liquefied. 



13. Streptococcus faecalis. 

 aa. Gelatin liquefied. 



14. Streptococcus liquejaciens. 



2. Beta hemolytic. 



a. Mannitol and sorbitol fermented. 



15. Streptococcus zymogenes. 

 aa. Mannitol and sorbitol not fermented. 



16. Streptococcals durans. 

 IL Anaerobic species. 



A. Strict anaerobes. 



1. Gas and fetid odor produced. 



a. No general turbidity in broth. 



