330 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



turbidity which does not precipitate 

 completely. No gas. Little odor. 



Martin glucose broth: Rapid abun- 

 dant growth. Uniform turbidity. Sedi- 

 ment. No gas. Slight fetid odor. 

 Black pigment in the sediment. 



Meat and liver broth: Very abundant 

 growth, very marked putrid odor. In- 

 complete sedimentation. 



Peptone water: Sparse growth. 

 Neither gas, odor, HoS nor indole. 



Milk: No acid. No coagulation. 



Cooked protein not attacked. 



Deep blood agar : Agar is broken by the 

 gas (HoS). 



Fresh blood broth : Abundant gas 

 which contains a large amount of H2S. 

 Blood blackens rapidly, has typical 

 putrid odor. 



Fresh fibrin broth : The fibrin is broken 

 up and partially digested. 



Neutral red changed to fluorescent 

 yellow. 



Lead media blackened. 



Acid from glucose, fructose and mal- 

 tose. Acid sometimes produced from su- 

 crose, mannitol and galactose. 



Optimum pH 7.0 to 8.5. 



Temperature relations : Optimum 36° 

 to 38°C. Growth feeble at 28°C. No 

 growth below 22°C. Killed in ten 

 minutes at 80°C. 



Pathogenic when grown in media with 

 fresh tissue and body fluids. 



Strict anaerobe. 



Distinctive characters : Putrescence 

 but absence of gas in ordinary media ; 

 presence of gas and H2S in media with 

 fresh tissue or body fluids. 



Source : Normal and fetid lochia, blood 

 in puerperal fever, gangrenous appendi- 

 citis, gangrene of the lung, in gas gan- 

 grene, gangrenous metastases; war 

 wounds; osteomyelitis; and from amni- 

 otic fluid. Found in sea water by Mon- 

 tel and Mousseron (Paris Medical, 1929 j. 



Habitat: Human mouth and intestine 

 and especially the vagina. 



20. Streptococcus lanceolatus Prevot. 

 (Coccus lanceolatus anaerobius Tissier, 



Compt. rend. Soc. i:ioL Paris, 94, 1926, 

 447; Pr6vot, Ann. Sci. Nat., Ser. Bot., 

 15, 1933, 173 and 193; not Streptococcus 

 lanceolatus pasteuri nor Streptococcus 

 lanceolatus Gamaleia, Ann. Inst. Past., 

 2, 1888, 440; not Streptococcus lanceolatus 

 Saito, Arch. f. Hyg., 75, 1912, 121.) 

 Although Prdvot's name is invalid, it is 

 used until further comparative studies 

 have been made. From Latin lanceolatus, 

 having a little lance, pointed. 



Large ovoid cells : 1 .2 to 1 .4 microns 

 with pointed ends, occurring in short 

 chains in culture and in pairs in exudates. 

 Non-motile. Gram-positive. 



Gelatin: No liquefaction. 



Deep agar colonies : Very large, lentic- 

 ular. Abundant gas produced which 

 breaks up the medium. 



Peptone broth: Uniform turbidity. 

 Granular, glairy sediment. 



Peptone water: Good growth. Gas 

 produced. 



Milk: No change. 



Protein not attacked. 



Hydrolyzed albumen reduced to CO2, 

 (NH4)2C03 and NH3. 



Acid from sucrose, glucose and starch. 

 No acid from lactose. (Butyric, valeri- 

 anic and acetic acid are produced, in the 

 proportions 2:1: trace, from glucose and 

 sucrose.) 



Non-pathogenic for laboratorj' animals. 



Optimum temperature 37°C. 



Strict anaerobe. 



Distinctive characters : Proteolytic 

 and saccharolytic ; produces ammonia 

 from hydrolyzed proteins; butyric, 

 valerianic and acetic acid produced from 

 hexoses. No H2S produced. 



Source : From human feces in a case of 

 diarrhoea. 



Habitat : Putrefying materials. 



21. Streptococcus micros Prevot. 

 (Streptococcus anaerobius micros Lewko- 

 wicz, Arch. Med. Exp., 13, 1901, 645; 

 Prevot, Ann. Sci. Nat., Ser. Bot., 15, 1933, 

 193; also see Weiss and Mercado, Jour. 

 Inf. Dis., 62, 1938, 181.) From Greek 

 micrus, small (old spelling, micros). 



