FAMILY LACTOBACTERIACEAE 



331 



Very small spheres : 0.2 to 0.4 micron, 

 occurring in long chains or in pairs. Xon- 

 motile. Gram-positive. 



Gelatin : Poor growth. No lique- 

 faction. 



Semi -solid agar (Veillon): Slow 

 growth; colonies at first punctiform, be- 

 coming lenticular and later forming proc- 

 esses into the medium. Average size 

 0.5 to 1.0 mm in diameter, some reach 

 2 to 3 mm growing 2 or 3 cm below the 

 surface. 



Blood agar: Xo hemolysis. Xo hemo- 

 peptolysis. 



Martin broth: Slight particulate tur- 

 biditj' which slowly settles. 



Meat and liver broth : Rapid growth. 

 Abundant sediment. 



Peptone water : Powdery sediment . 

 ^Medium not acidified. X"o indole formed. 



Xeutral red broth : Changed to fluores- 

 cent yellow. 



Milk : Grows with difiicult3\ Xo acid. 

 Xo coagulation. 



Acid produced rapidly from glucose, 

 fructose, galactose, sucrose and maltose. 

 Xo acid from lactose, arabinose, glycerol, 

 mannitol, inulin and starch. 



Protein not attacked. 



Optimum pH about 7.0. 



Optimum temperature 36° to 38°C. 

 Xo growth at 22°C. Killed in a quarter 

 of an hour at 60°C. 



Non-pathogenic for mice. 



No toxin and no hemolj'sin. 



Strict anaerobe. 



Distinctive characters : Neither gas 

 nor fetid odor produced. Small size. 



Source: Gangrene of the lung; lochia 

 and uterus in puerperal sepsis; ap- 

 pendicitis. 



Habitat : Mouth and intestine of man 

 and animals. 



22. Streptococcus parvulus Weinberg, 

 Nativelle and Prevot. {Streptococcus 

 parvulus non liquefaciens Repaci, Compt. 

 rend. Soc. Biol., Paris, 68, 1910, 528; 

 Weinberg, Nativelle and Prevot, Les 

 Microbes Anaerobies, 1937, 1011; not 

 Streptococcus parvulus Levinthal, Cent. 



f. Bakt., I Abt., Orig., 106, 1928, 195.) 

 From Latin parvulus, very small, minute. 



Small spheres : Average size 0.3 to 0.4 

 micron, occurring in short chains, some- 

 times in pairs. Xon-motile. Gram- 

 positive. 



Gelatin : At 37°C slow growth, culture 

 at bottom of the tube ; no gas. Xo lique- 

 faction. 



Deep glucose agar colonies : After 48 

 hours very tinj% lenticular, whitish. Old 

 colonies become blackened. Xo gas pro- 

 duced. 



Broth: Rapid turbidity. Sediment 

 forms in 5 or 6 days as a whitish, mucous 

 mass which clears the fluid. Xo gas. 

 Faint disagreeable odor. 



Indole not formed. 



Milk: Coagulation in 24 hours. 



Egg white not attacked. 



Feeblj' attacks glucose and lactose. 

 Does not attack sucrose, galactose and 

 de.xtrin. 



Optimum temperature 37°C. Xo 

 growth at room temperature. Will grow 

 at 41 °C. 



Strict anaerobe. 



Distinctive characters ; Differs from 

 Streptococcus tnicros by its black colonies, 

 coagulation of milk and its feeble sac- 

 charolytic power. Differs from Strepto- 

 coccus intennedius by its black colonies, 

 the smallness of its elements, feeble sac- 

 charolytic power and the viscous sedi- 

 ment in broth. 



Source: Respirator}- tract. 



Habitat: Unknown. 



^'eillon and Repaci identified this or- 

 ganism as Streptococcus micros, but 

 Weinberg, Xativelle and Prevot consider 

 it as a distinct species, although rare. 



23. Streptococcus intermedins Prevot. 

 (Ann. Inst. Past., 39, 1925, 439.) From 

 Latin inter medius, intermediate. 



Description taken in part from Prevot, 

 Ann. Sci. Xat., Ser. Bot., 15, 1933, 197. 



Spheres: 0.5 to 0.7 micron, very long 

 chains in culture. Xon-motile. Gram- 

 positive. 



