FAMILY LACTOBACTERIACEAE 



333 



ternate zones in agar. Strict anaerobe 

 at first, later microaerophilic. 



Source: Skin abscess; appendicitis. 



Habitat: Respiratory tract, mouth, 

 vagina. 



Appendix I: Descriptions of poorly de- 

 fined species, the taxonomic relation- 

 ships of which are not clear. 



1. Streptococcus sp. Long and Bliss. 

 (Minute beta hemolytic streptococcus, 

 Long and Bliss, Jour. Exp. Med., 60, 

 1934, 619; Long, Bliss and Walcott, ibid., 

 633.) 



Minute cocci, half to two-thirds the size 

 of Streptococcus pyogenes; occurring 

 singly, in pairs, short chains and in small 

 and large masses. Gram-positive, but 

 may decolorize readily. 



Blood agar: Very minute colonies 18 

 to 30 microns, surrounded by a marked 

 area of hemolysis (beta), easily visible 

 before the colony is seen by naked eye, 4 

 to 10 times the diameter of the colony. 

 Under the microscope colonies are finely 

 granular, may appear wrinkled and 

 crenated. Colonies become visible after 

 48 to 96 hours incubation and relative 

 area of hemolysis is 3 to 4 times diameter 

 of colony. 



Gelatin: Xot liquefied. 



Glucose broth: Growth diffuse, 

 abundant. 



Litmus milk: Not curdled; litmus not 

 reduced. 



Acid from glucose, maltose and su- 

 crose; may or may not attack lactose, 

 trehalose and salicin. No acid from 

 arabinose, raffinose, inulin. glycerol, man- 

 nitol or sorbitol. 



Does not hydrolyze sodium hippurate 

 and starch. Plsculin is hydrolyzed. 



Ammonia is produced from peptone. 



Temperature relations : No growth at 

 10°C, very rarely growth at 45°C. Does 

 not survive 60°C' for 30 minutes. 



Chemical tolerance : Does not tolerate 

 6.5 per cent NaCl. Final pH in glucose 

 broth 5.4 to 4.6; no growth at pH 9.6. 

 Methylene blue 0.1 per cent not tolerated. 

 No growth on 40 per cent bile-blood agar. 



Action on blood: Hemolysis marked 

 before colony is visible. May not pro- 

 duce soluble hemolj'sin by ordinary meth- 

 ods but does so abundantly by appropriate 

 methods. 



Fibrinolysin : No solution of human 

 fibrin. 



Serology : Constitutes Group F of 

 Lancefield and Hare (Jour. Exp. Med., 

 67, 1935, 335). Four serological types 

 within the group (Bliss, Jour. Bact., 33, 

 1937, 625). 



Aerobe, facultative anaerobe. 



Source : Human throat in health and 

 disease, accessory sinuses, abscesses, 

 vagina, skin and feces. 



Habitat : Human upper respiratory 

 tract. 



2. Streptococcus sp. Long and Bliss. 

 (Group II, Long and Bliss, Jour. Exp. 

 Med., 60, 1934, 633; Group G, Lancefield 

 and Hare, Jour. Exp. Med., 61, 1935, 

 346; Bliss, Jour. Bact., 83, 1937, 625.) 



Probably identical with Streptococcus 

 anginosus Andrewes and Horder (Lancet, 

 2, 1906, 712) but probably other serolog- 

 ical types are included in this group 

 (Sherman, Bacteriological Reviews, 1, 

 1937, 40). 



Spheres : Gram -positive. 



Gelatin: Not liquefied. 



Litmus milk: Acid, may be curdled, 

 not reduced. 



Acid from glucose, maltose, sucrose, 

 trehalose and salicin; usually acid from 

 lactose, and may or may not from raffi- 

 nose and glj^cerol. No acid from arab- 

 inose, inulin, mannitol or sorbitol. 



Sodium hippurate usually not hydro- 

 lyzed. May hydrolyze starch and 

 esculin. 



Ammonia is produced from peptone. 



Temperature relations: No growth at 

 10°C and usually not at 45°C. Does not 

 survive 60 ''C for 30 minutes. 



Chemical tolerance: Does not tolerate 

 6.5 per cent NaCl. Final pH in glucose 

 broth 6.0 to 4.6; no growth at pU 9.6. 

 Methylene blue 0.1 per cent not tol- 



