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MANUAL OF DETERMINATIVE BACTERIOLOGY 



erated. May grow on 40 per cent bile- 

 blood agar, growth in 10 per cent bile. 



Action on blood: Hemolytic (beta) 

 with a wider zone than minute beta 

 hemolytic streptococcus. Soluble hemol- 

 ysin formed. 



Fibrinolysis : May dissolve human 

 fibrin, certain strains strongly, others 

 weakly. 



Serology : Constitutes Lancefield's and 

 Hare's Group G. Bliss (loc. cit.) has 

 shown serological Types I and II within 

 the group. May include serological Type 

 16 of Griffith (Jour. Hyg., 34, 1934, 

 542). Those resembling Streptococcus 

 anginosus seem to form a homogeneous 

 type ; others seem unrelated to it. 



Aerobic, facultative anaerobe. 



Source : Human nose, throat, vagina, 

 skin and feces in health. In human dis- 

 ease, in puerperal fever with staphylo- 

 coccus. Throat of normal domestic ani- 

 mals and in animal infections probably 

 as secondary invaders. 



Habitat: Human upper respiratory 

 tract and vagina. Possibly throat of 

 domestic animals. 



3. Streptococcus sp. Brown, Frost and 

 Shaw. (Jour. Inf. Dis., 38, 1926, 381; 

 Lancefield, Jour. Exp. Med., 57, 1933, 

 571.) 



Belongs to Lancefield Group E. 



Gelatin: Not liquefied. 



Litmus milk : Not curdled and not re- 

 duced. 



Acid from glucose, lactose, trehalose 

 and sorbitol ; may form acid from sucrose, 

 glycerol, mannitol and salicin. No acid 

 from arabinose, raffinose or inulin. 



No hydrolysis of sodium hippurate; 

 may hydrolyze starch and esculin. 



Ammonia is produced from peptone. 



Temperature relations : No growth at 

 10°C and 45°C. Does not survive 60°C 

 for 30 minutes. 



Chemical tolerance : Does not tolerate 

 6.5 per cent NaCl. Final pH in glucose 

 broth 4.8 to 4.2; no growth at pH 9.6. 

 Methylene blue 0.1 per cent not tolerated 



and not reduced. No growth on 40 per 

 cent bile-blood agar, nor on 10 per cent bile. 



Action on blood : Very hemolytic ; 

 strains reported by Plastridge and 

 Hartsell (Jour. Inf. Dis., 61, 1937, 110) 

 weakly hemolytic. Streptolysin pro- 

 duced and outstandingly acid stable 

 (Todd, Jour. Path, and Bact., 39, 1934, 

 299). 



P'ibrinolysin : No solution of human 

 fibrin. 



Serology: Lancefield Group E, some 

 cross reaction with Group C. 



Aerobe, facultative anaerobe. 



Source: Certified milk; bovine udder. 



Habitat : Probably in udder and dairy 

 products. 



4. Streptococcus sp. Hare. (Group 

 H, Hare, Jour. Path, and Bact., 4^, 

 1935, 499.) 



Spheres : Gram-positive. 



Blood agar: Small colonies, 0.7 to 0.9 

 mm, smooth surface, greenish color tend- 

 ing to blacken, hard, almost gritty and 

 adherent to the medium. Hemolysis 

 seldom complete except on Brown's 

 horse blood agar. On boiled blood agar 

 definite greening and so different from 

 Groups E, FandK. 



Litmus milk: Not curdled and not 

 reduced. 



Acid from glucose, maltose, sucrose, 

 raffinose and salicin ; acid may be formed 

 from lactose and trehalose. No acid from 

 arabinose, inulin, glycerol, mannitol or 

 sorbitol. 



No hydrolysis of sodium hippurate and 

 starch, but may hydrolyze esculin. 



Ammonia may or may not be produced 

 from peptone. 



Temperature relations : No growth at 

 10°C. Growth at 45°C. May survive 

 60°C for 30 minutes. 



Chemical tolerance : Does not tolerate 

 6.5 per cent NaCl. Final pH in glucose 

 broth 5.0 to 4.5; no growth at pH 9.6. 

 Methylene blue 0.1 per cent not tolerated. 

 No growth on 40 per cent bile-blood agar. 



Action on blood : Hemolysis incomplete 



