352 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Minimum 18° to 22°C. Maximum 50°C'. 



Source : From milk and cheese. 



Habitat : Undoubtedly widely distrib- 

 uted in milk or milk products. 



3. Lactobacillus helveticus (Orla-Jen- 

 sen) Holland. {Bacillus e, von Freuden- 

 reich, Cent. f. Bakt., II Abt., 1, 1895, 

 173; also Landw. Jahrb. d. Schweiz, 1895, 

 211 ; BacUbis casei e, v. Freudenreich and 

 Thoni, Landw. Jahrb. d. Schweiz, 1904, 

 526; also Orla-Jensen, Cent. f. Bakt., II 

 Abt., 13, 1904, 609; Caseobacterhmi e, 

 Orla-Jensen, Cent. f. Bakt., II Abt., 

 22, 1909, 337; Thermobacterivm helveti- 

 cwn Orla-Jensen, Maelkeri-Bakteriologie, 

 1916, 35; also the Lactic Acid Bacteria, 

 1919, 164; Bacterium casei e, Holland, 

 Jour. Bact., 5, 1920, 221; Holland, ibid., 

 223.) From Latin helveticus, Swiss. 



Rods: 0.7 to 0.9 by 2.0 to 6.0 microns, 

 occurring singly and in chains. Non- 

 motile. Gram-positive. 



Whey gelatin colonies : Does not grow 

 readily at temperatures required for 

 incubation of gelatin. 



Lactose agar colonies: Small, grayish, 

 viscid. 



Milk: Acid, with coagulation; may 

 become slimy. 



Nitrites not produced from nitrates. 



Acid from glucose, fructose, galactose, 

 mannose, maltose, lactose, and smaller 

 amounts from dextrin. The lactic acid 

 is inactive. 



Temperature relations : Optimum 40° 

 to 42°C. Minimum 20° to 22°C. Maxi- 

 mum 50°C. 



Microaerophilic . 



Source : From sour milk and cheese. 



Habitat: Widely distributed in dairy 

 products. 



4. Lactobacillus acidophilus (Moro) 

 Holland. {Bacillus acidophilus Moro, 

 Wiener klin. Wochnschr., 13, 1900, 114; 

 also Jahrb. f . Kinderheilkunde, 52, 1900, 

 38; Holland, Jour. Bact., 5, 1920, 215; 

 Plocamobacterium acidophilum Lehmann 

 and Neumann, Bakt. Diag., 7 Aufl., 2, 

 1927, 510; Thermobacterium intestinale 



Orla-Jensen, Orla-Jensen and Winther, 

 Cent. f. Bakt., II Abt., 93, 1936, 321.) 

 From Latin acidus, sour; M. L. acidus, 

 acid and Greek philus, loving. 



Possible synonyms : Milchsaurebacil- 

 lus, Boas and Oppler, Deutsche med. 

 Wochnschr., 21, 1895, 73; Diagnostik und 

 Therapie d. Magenkrankheiten, II Teil, 

 1907, 265 {Lactobacillus boas-oppleri 

 Bergeyetal., Manual, 1st ed., 1923,243); 

 Bacilbis exilis Tissier, La flore intestinale 

 des nourrissons, Paris, 1900, 102; Bacillus 

 gastrophilus Lehmann and Neumann, 

 Bakt. Diag., 4 Aufl., 2, 1907, 424 {Bac- 

 terium gastrophilum Lehmann and Neu- 

 mann, Bakt. Diag., 5 Aufl., 2, 1912, 305) ; 

 Bacillus acetogenus a Distaso, Cent. f. 

 Bakt., I Abt., Orig., S9, 1911, i9; Bacillus 

 acetogenus j8 Distaso, ibid., 51; Bacillus 

 acetogenus proteiformis Distaso, ibid., 52; 

 Bacillus acetogenus exilis Distaso, ibid., 

 53; Bacillus paraexilis Distaso, ibid., 66; 

 Bacillus dimnrphus Distaso, ibid., 55; 

 Bacillus dimorphus var. longa Distaso, 

 Cent. f. Bakt., I Abt., Orig., 62, 1912, 

 440; {Bacteroides dimorphus Bergey et al., 

 Manual, 1st ed., 1923, 258); Streptobacil- 

 lus long us Distaso, ibid., 439; Thermo- 

 bacterium acidophilum Henneberg, Cent. 

 f. Bakt., II Abt., 91, 1934, 102. 



Description of Moro supplemented by 

 material from Kulp and Eettger, Jour. 

 Bact., 9. 1924, 357; Curran, Rogers and 

 Whittier, Jour. Bact., 25, 1933, 595; and 

 Rcttger, Levy, Weinstein and Weiss, 

 Lactobacillus acidophilus, Yale Univ. 

 Press, New Haven, 1935. 



Rods : 0.6 to 0.9 by 1.5 to 6.0 microns, 

 occurring singly, in pairs and in short 

 chains with rounded ends. Non-motile. 

 Dimensions variable (Kulp and Rettger), 

 (Curran, Rogers and Whittier). Gram- 

 positive; old cultures often Gram-nega- 

 tive (Moro). 



Gelatin : No growth at 20°C. No lique- 

 faction. 



Wort-agar (Moro ) or tomato agar (Kulp 

 and Rettger) plates: Surface colonies, 

 peripheries a capilliform maze of long, 

 delicate, twisted, fuzzy projections, cen- 

 ter appears as a thick, dark, felt-like 



