376 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Probablj' from silage. 



Habitat : Dairy products. 



6. Propionibacterium peterssonii van 



Niel. {Bacterium acidi propionici c, 

 Troili-Petersson, Cent. f. Bakt., II Abt., 

 24, 1909, 333; van Niel, The Propionic 

 Acid Bacteria, 1928, 163; Werkman and 

 Brown, Jour. Bact., 26, 1933, 406.)Named 

 for Gerda Troili-Petersson, the Swedish 

 bacteriologist, who isolated this or- 

 ganism. 



Description taken from van Xiel, and 

 Werkman and Brown. 



Cells in neutral media spherical, O.S 

 micron, occurring as short streptococci 

 in clumps. In carbohydrate media which 

 turn acid during development, rod-shaped 

 cells in clumps, 0.8 by 1.5 to 2.0 microns. 

 Aerobic growth, heavily swollen and 

 branched rods. Non -motile. Show 

 metachromatic granules. (iram-iiosi- 

 tive. 



Yeast gelatin-lactate stab : No lique- 

 faction. 



Yeast agar-lactate stab : Cream-colored 

 growth, dry and wrinkled, resembling 

 that of Mycobacterium spp. 



Liquid media: No turbidity, sediment 

 a coherent layer, cream-colored. 

 Litmus milk: Acid, coagulated. 

 Catalase positive; aerobically de- 

 veloped growth very slightly so. 

 Indole not formed. 



Nitrites not produced from nitrates. 

 Ferments lactic and pyruvic acids, 

 glycerol, dihydroxyacetone, glucose, fruc- 

 tose, mannose, galactose, sucrose, malt- 

 ose and lactose with the formation of 

 propionic and acetic acids, and carbon 

 dioxide. 



Acid from esculin and salicin. No acid 

 from d- and 1-arabinose, cellobiose, dex- 

 trin, dulcitol, glycogen, inulin, perseitol, 

 pectin, raffinose, rhamnose, sorbitol, 

 starch or xylose. 



Less anaerobic than Propionibacterium 

 frcudenreichii and Propionibacterium 

 shermanii. 



Distinctive character: Growth in 

 liquid media in clumps, giving the cui 



tures the appearance of agglutinated 

 bacteria. So far, the only species among 

 the propionic acid bacteria possessing 

 this characteristic. 



Source : From cheese and soil. 



Habitat: Dairy products. 



7. Propionibacterium jensenii van 

 Niel. {Bacterium acidi propionici b, 

 von Freudenreich and Orla- Jensen, Cent, 

 f. Bakt., II Abt., 17, 1906, 532; van Niel, 

 The Propionic Acid Bacteria, 1928, 163; 

 Werkman and Brown, Jour. Bact., 26, 

 1933, 404.) Named for Prof. S. Orla- 

 Jensen, the Danish bacteriologist, who 

 isolated this organism. 



Description taken from van Niel, and 

 Werkman and Brown. 



In neutral media spherical to short 

 rod-shaped cells, often in pairs or short 

 chains, 0.8 by 0.8 to 1.5 microns, of typi- 

 cal diphtheroid appearance. Morphology 

 little influenced by developing acidity. 

 Aerobic growth, irregular long rods, 

 swollen and branched. Non-motile. 

 Metachromatic granules. Gram-posi- 

 tive. 



Yeast gelatin-lactate stab: No lique- 

 faction. 



Yeast agar-lactate stab : Cream-colored 

 growth in stab, orange-yellow, dome- 

 shaped surface growth. 



Liquid media: Turbid in early stages; 

 cream-colored, smooth sediment. 



Litmus milk: Coagulated, acid. 



Catalase: Strongly ixisitive. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ferments lactic and pyruvic acids, 

 glycerol, dihydroxj^acetone, glucose, fruc- 

 tose, mannose, galactose, sucrose, malt- 

 ose, lactose and sometimes raffinose and 

 mannitol with the formation of propionic 

 and acetic acids, and carbon dioxide. 



Acid from adonitol, arabitol, erythritol, 

 esculin, inositol and trehalose. No acid 

 from arabinose, cellobiose, dextrin, dulci- 

 tol, glycogen, inulin, perseitol, pectin, 

 rhamnose, salicin, sorbitol, starch or 

 xvlose. 



