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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Broth: Slightly turbid with slight, 

 grayish sediment. 



Litmus milk: Unchanged. 



Potato : Slight, creamy-white, smooth, 

 entire growth. 



Indole not formed. 



Nitrites produced from nitrates. 



No acid from carbohydrate media. 



Hydrolyzes urea (Merkel, Cent. f. 

 Bakt., I Abt., Orig., U7, 1941, 398). 



Aerobic, facultative. 



Optimum temperature 37°C. 



Not pathogenic. 



Source : From oral cavity of 26 out of 

 45 control cases. 



Habitat: Normal throats. 



3. Corynebacterium enzymicum (Mel- 

 lon) Eberson. (An unusual diphtheroid 

 bacillus, Mellon, Med. Record, New 

 York, 81, 1916, 240; Bacillus enzymicus 

 Mellon, Jour. Bact. , 2, 1917, 297 ; Eberson, 

 Jour. Inf. Dis., 23, 1918, 29.) From 

 Greek en, inside of; zyme, leaven; of an 

 enzyme. 



Rods, beaded and club-shaped, defi- 

 nitely pleomorphic , showing coccoid forms . 

 Non-motile. Gram-positive. 



Gelatin stab : Slight surface growth. 

 No liquefaction. 



Glucose agar : Bacillary form shows 

 very small colorless colonies. Coccoid 

 form shows heavy, yellowish-white, moist 

 growths. 



Blood agar : Same as on glucose agar. 



Loeffler's blood serum: Fine, moist, 

 confluent growth. 



Glucose broth : Bacillary form shows 

 granular sediment. Coccoid form shows 

 diffuse, luxuriant growth. 



Litmus milk: Acid, coagulated. 



Potato : No growth. 



Indole formation slight. 



Slight production of nitrites from ni- 

 trates. 



Acid from glucose, maltose, sucrose, 

 dextrin and glycerol. 



Aerobic, facultative. 



Optimum temperature 37°C. 

 \ Pathogenic for rabbits, guinea pigs and 

 mice. 



Source: Lungs, blood and joints. 

 Habitat : From human sources so far as 

 known. 



4. Corynebacterium xerose (Neisser 

 and Kuschbert) Lehmann and Neumann. 

 {Bacillus xerosis Neisser and Kusch- 

 bert, Breslauer artzl. Ztschr., No. 4, 

 1883; Xerosebacillen, Kuschbert, 

 Deutsche med. Wochnschr., 10, 1884, 

 321 and 341 ; Pacinia neisseri Trevisan, 

 I generi e le specie delle Batteriacee, 

 1889, 23; Lehmann and Neumann, Bakt. 

 Diag., 2 Aufl., 2, 1899, 405; Bacterium 

 xerosis Migula, Syst. d. Bakt., 2, 1900, 

 485.) From Greek xerus, dry. 



An excellent historical discussion of 

 this organism is given by Andrewes et al.. 

 Diphtheria. London, 1923, 377-382. 



Rods, showing polar staining, occa- 

 sionally club-shaped forms are seen. 

 Non-motile. Gram-positive. 



Plain gelatin colonies: Rarely develop. 



Serum gelatin stab: No liquefaction. 



Agar colonies: Minute, circular, al- 

 most transparent, raised, smooth, pearly 

 white. 



Agar slant : Thin, grayish, limited 

 growth. 



Loeffler's blood serum: Thin, grayish, 

 adherent growth. 



Broth: Clear, with slight, granular 

 sediment. 



Litmus milk : Unchanged. 



Potato : No visible growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid from glucose, fructose, galactose, 

 maltose and sucrose. 



Not pathogenic. 



Aerobic, facultative. 



Optimum temperature 37 °C. Grows 

 veryslowlyaslowasl8°to25°C (Eberson, 

 Jour. Inf. Dis., 23, 1918, 3). 



Source : From normal and diseased con- 

 junctiva. 



Habitat : Probably identical with other 

 species described from the skin and 

 other parts of the body. 



