388 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



terium acnes, and eleven species listed by 

 Pr^vot (Manual de Classification et de 

 Determination des Bacteries Anaerobies. 

 Monographic, Inst. Past., Paris, 1940, 

 199-204) as follows: Corynebacterium 

 diphtheroides, C. avidum, C. renale cuni- 

 culi, C. lymphuphilmn, C. hepatodystro- 

 phicans, C. parvum, C. anaerohium, C. 

 granulosurn, C. adamsoni, C. liquefaciens, 

 and C. pyogenes bovis. 



7. Corynebacterium pyogenes (Glage) 

 Eberson. {Bacillus liquejaciens pyo- 

 genes bovis Lucet, Ann. Inst. Past. 7, 

 1893, 327; Bacillus liquefaciens pyogenes 

 Lucet, ibid., 327; Bacillus liquefaciens 

 Lucet, ibid.; Bakterium der multipler 

 Abszessbildung der Schweine, Grips, 

 Ztschr. f. Fleisch- u. Milchhyg., 8, 1898, 

 166; Bacillus pyogenes bovis Kiinnemann, 

 Arch. f. wiss. u. prakt. Tierheilk., 29, 

 1903, 128; Bacillus pyogenes Glage, 

 Ztschr. f . Fleisch- u. Milchhyg., 13, 1903, 

 166; not Bacillus pyogenes Lucet, Ann. 

 Inst. Past., 7, 1893, 327; Bacillus pyo- 

 genes suis Lehmann and Neumann, Bakt . 

 Diag., 4 Aufl.,^, 1907, SM; Bacterium hyo- 

 pyogenes Lehmann and Neumann, Bakt. 

 Diag., 4 Aufl., 2, 1907, 394; Bacterium 

 pyogenes suis Lehmann and Neumann, 

 Bakt. Diag., 7 Aufl., 2, 1927, 499; Bac- 

 terium pyogenes Ward, Jour. Bact., 2, 

 1917, 519; not Bacterium pyogenes Ches- 

 ter, Man. Determ. Bact., 1901, 184; 

 Eberson, Jour. Inf. Dis., 23, 1918, 5; 

 not Corynebacterium pyogenes Lewand- 

 owsky. Cent. f. Bakt., I Abt., Orig., 

 36, 1904, 473; Corynebacterium pseu- 

 dopyogenes Ochi and Zaizen, Jour. Jap. 

 Soc. Vet. Sci., 15, 1936, 12 and 16, 

 1937, 8.) From Greek pyum, pus; 

 gignomai, producing. 



For description see Brown and Orcutt, 

 Jour. Exp. Med., 32, 1920, 244. 



Rods : 0.2 by 0.3 to 2 microns in length. 

 Smallest forms appear as scarcely visible 

 points (common in old abscesses). 

 Chains formed. Club forms may be 

 present. Non-motile. Gram-positive. 



Serum gelatin: Liquefaction. 



No growth on ordinary agar. 



Serum agar: Minute colonies after 36 



to 48 hours. Surface colonies may in- 

 crease to 3 mm in diameter. Colonies 

 smoky brown by transmitted light and 

 bluish-white by reflected light. 



Bovine blood serum slants : Pit-like 

 or more general areas of liquefaction. 



Serum bouillon : Cloudy with fine floc- 

 culent grayish flakes that form a sedi- 

 ment like a streptococcus culture. 



Milk: Coagulation after 48 hours 

 at 37°C, with acid at bottom of tube. 

 Separation of whey and peptonization. 



Nitrites not produced from nitrates 

 (Merchant, Jour. Bact., 30, 1935, 108). 



Indole not formed. 



Acid formed in serum bouillon from 

 glucose, sucrose, lactose, and xylose but 

 not from raffinose, inulin, mannitol and 

 salicin. 



Beta hemolytic, not hemoglobino- 

 philic though growth is favored by pro- 

 teins as egg albumen, serum or blood 

 (Brown and Orcutt, loc. cit.). 



Optimum temperature 37°C. Growth 

 range 20° to 40 °C. 



Intravenous injection of rabbits fatal. 



Aerobic as well as anaerobic growth. 



Source: Fi'om bovine pus. 



Habitat : Found in abscesses in cattle, 

 swine and other domestic animals. 



8. Corynebacterium renale (Migula) 

 Ernst. {Bacillus renalis bovis Bollinger, 

 in Enderlen, Zeit. f. Tiermed., 17, 1890, 

 346; Bacillus pyelonephritis bourn (sic) 

 Hoflich, Monatsh. f . prakt. Tierheilk., 2, 

 1891, 356; Bacterium renale Migula, 

 Syst. d. Bakt., 2, 1900, 504; Bacillus 

 renalis Ernst, Cent. f. Bakt., I Abt., 

 Orig., 39, 1905, 550; Ernst, ibid., 40, 1905, 

 80; Coryncbacteriiun renalis bovis Ernst, 

 ibid., 82.) From Latin renalis, kidney. 



Description largely taken from Jones 

 and Little, Jour. Exp. Med., U, 1926, 11. 



Rods : 0.7 by 2 to 3 microns. Non- 

 motile. Usually in masses, rarely single. 

 Bacteria from tissues not as pleomorphic 

 as those from the earlier transfer cul- 

 tures although many show polar granules 

 or swollen ends. Cultures grown in broth 

 show coccoid forms and beaded rods with 

 swollen ends. Gram-positive. 



